BisEDT and RIP act in synergy to prevent graft infections by resistant staphylococci

Staphylococci are a major cause of infections associated with indwelling medical devices. Biofilm formation on these devices adds to the antibiotic resistance seen among clinical isolates. RNAIII-inhibiting peptide (RIP) is a heptapeptide that inhibits staphylococcal pathogenesis, including biofilm...

Full description

Saved in:
Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2004-12, Vol.25 (12), p.2047-2053
Main Authors: Domenico, Philip, Gurzenda, Ellen, Giacometti, Andrea, Cirioni, Oscar, Ghiselli, Roberto, Orlando, Fiorenza, Korem, Moshe, Saba, Vittorio, Scalise, Giorgio, Balaban, Naomi
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antimicrobial peptides
Biofilms
Biofilms - drug effects
Biological and medical sciences
Bismuth thiols
Drug Resistance, Bacterial
Drug Synergism
Implants, Experimental - adverse effects
Male
Medical sciences
Mercaptoethanol - analogs & derivatives
Mercaptoethanol - therapeutic use
Models, Biological
Oligopeptides - therapeutic use
Pharmacology. Drug treatments
Polyethylene Terephthalates
Rats
Rats, Wistar
Rifampin - therapeutic use
RNAIII inhibiting peptide
Staphylococcal Infections - drug therapy
Staphylococcal Infections - prevention & control
Staphylococci
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus epidermidis
Staphylococcus epidermidis - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Staphylococci are a major cause of infections associated with indwelling medical devices. Biofilm formation on these devices adds to the antibiotic resistance seen among clinical isolates. RNAIII-inhibiting peptide (RIP) is a heptapeptide that inhibits staphylococcal pathogenesis, including biofilm formation, by obstructing quorum sensing mechanisms. Bismuth ethanedithiol (BisEDT) also prevents biofilm formation at subinhibitory concentrations. RIP and BisEDT were combined to prevent infections in a rat graft model, using antibiotic sensitive and resistant strains of Staphylococcus aureus and Staphylococcus epidermidis. BisEDT, RIP, or rifampin, or their combinations reduced the graft associated bacterial load over seven days. BisEDT–RIP was the best combination, reducing bacterial load to undetectable levels. BisEDT–RIP may prove useful for coating medical devices to prevent staphylococcal infections.
ISSN:0196-9781
1873-5169
DOI:10.1016/j.peptides.2004.08.005