Effects of experimental terbuthylazine exposure on the cells of Dicentrarchus labrax (L.)

• Published in: Chemosphere (Oxford) 2006-09, Vol.64 (10), p.1684-1694
• Main Authors: Dezfuli, B.S., Simoni, E., Giari, L., Manera, M.
• Format: Article
• Language: English
• Subjects: acute toxicity; Agnatha. Pisces; Analysis of Variance; Animal, plant and microbial ecology; Animals; Applied ecology; Bass; Biological and medical sciences; Biometry; Chloride cells; cytotoxicity; Dicentrarchus labrax; Ecotoxicology, biological effects of pollution; Environmental Exposure; Fundamental and applied biological sciences. Psychology; General aspects; gills; Gills - drug effects; herbicide residues; intestines; Intestines - drug effects; Kidney - drug effects; kidneys; Liver - drug effects; Macrophage aggregates; Marine; Microscopy, Electron; Rodlet cells; Terbuthylazine; Triazines - toxicity; ultrastructure; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Water Pollutants, Chemical - toxicity
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2006.01.023

The effects of acute exposure to the herbicide terbuthylazine (3.55, 5.01 and 7.08 mg l −1) on the cells of farmed European sea bass, Dicentrarchus labrax L., were investigated by means of light and electron microscopy. In gills of treated fish, the number of chloride cells (CCs) and rodlet cells (RCs) increased significantly within 24 h and 48 h, respectively; the intestine showed the largest increase in RCs linked to treatment and exposure time. In kidney, 24 h exposure induced a significant increase in RCs and the number and global area of macrophage aggregates (MAs). Treated fish displayed cellular and/or ultrastructural alterations in all the organs examined. In the gills necrosis, lamellar and cellular oedema, epithelial lifting, telangectasia, and fusion of secondary lamellae were encountered. The liver presented myelin-like figures, cytoplasmic rarefaction and acute cell swelling of hepatocytes. In both organs, the severity of damage was dose–dependent. In RCs of gills, the intestine and kidney of exposed sea bass, high cytoplasmic vacuolization, myelin-like figures, cristolysis and varying degrees of rodlet degeneration were observed. Extensive rodlet expulsion occurred in the gut lumen. Exposure to terbuthylazine also affected the renal tubular epithelial cells, which exhibited ‘blebs’. Damage to the intestinal epithelial cells was also observed.