The heterologous expression strategies of antimicrobial peptides in microbial systems

• Published in: Protein expression and purification 2017-12, Vol.140, p.52-59
• Main Authors: Deng, Ting, Ge, Haoran, He, Huahua, Liu, Yao, Zhai, Chao, Feng, Liang, Yi, Li
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - therapeutic use; Anti-Infective Agents - chemistry; Anti-Infective Agents - therapeutic use; Antimicrobial Cationic Peptides - biosynthesis; Antimicrobial Cationic Peptides - chemistry; Antimicrobial Cationic Peptides - genetics; Antimicrobial Cationic Peptides - therapeutic use; Antimicrobial peptides; Bacillus subtilis - genetics; Drug Resistance, Microbial - genetics; Escherichia coli - genetics; Expression strategy; Heterologous expression; Humans; Pichia - genetics; Saccharomyces cerevisiae - genetics
• ISSN: 1046-5928; 1096-0279
• DOI: 10.1016/j.pep.2017.08.003

Antimicrobial peptides (AMPs) consist of molecules acting on the defense systems of numerous organisms toward tumor and multiple pathogens, such as bacteria, fungi, viruses, and parasites. Compared to traditional antibiotics, AMPs are more stable and have lower propensity for developing resistance through functioning in the innate immune system, thus having important applications in the fields of medicine, food and so on. However, despite of their high economic values, the low yield and the cumbersome extraction process in AMPs production are problems that limit their industrial application and scientific research. To conquer these obstacles, optimized heterologous expression technologies were developed that could provide effective ways to increase the yield of AMPs. In this review, the research progress on heterologous expression of AMPs using Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisiae as host cells was mainly summarized, which might guide the expression strategies of AMPs in these cells. •The classification and functional mechanisms of current AMPs are introduced.•The heterologous expression levels of AMPs in various microorganisms are presented.•The effective strategies for AMPs expression in various host cells are summarized.