Novel surface polypeptides of Campylobacter jejuni as traveller's diarrhoea vaccine candidates discovered by proteomics

Campylobacter jejuni is one of the most common causes of traveller's diarrhoea and food poisoning, therefore development of a vaccine is important. Using biochemical fractionation and mass spectrometry analysis, we identified more than 110 surface polypeptides. Eight C. jejuni identified surfac...

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Bibliographic Details
Published in:Vaccine 2006-09, Vol.24 (40), p.6446-6455
Main Authors: Prokhorova, T.A., Nielsen, P.N., Petersen, J., Kofoed, T., Crawford, J.S., Morsczeck, C., Boysen, A., Schrotz-King, P.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Antigens, Surface - immunology
Antigens, Surface - isolation & purification
Antigens, Surface - metabolism
Applied microbiology
Bacterial Proteins - immunology
Bacterial Proteins - isolation & purification
Bacterial Proteins - metabolism
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Campylobacter jejuni
Campylobacter jejuni - immunology
Campylobacter jejuni - metabolism
Diarrhea - immunology
Diarrhea - prevention & control
Escherichia coli
Fundamental and applied biological sciences. Psychology
Mice
Microbiology
Miscellaneous
Peptides - immunology
Peptides - isolation & purification
Peptides - metabolism
Protein vaccine
Proteins
Proteomics
Travel
Traveller's diarrhoea
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Campylobacter jejuni is one of the most common causes of traveller's diarrhoea and food poisoning, therefore development of a vaccine is important. Using biochemical fractionation and mass spectrometry analysis, we identified more than 110 surface polypeptides. Eight C. jejuni identified surface proteins were expressed in Escherichia coli and purified. Mice were immunized with different doses of these purified proteins and challenged orally with C. jejuni strains ML1 and ML53. The degree of protection of mice was tested by intestinal colonization. At least two groups of mice vaccinated with purified proteins clear the infection faster than control mice. Here, we present the use of a proteomics based approach for the identification of novel protein based C. jejuni vaccines for the first time.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2006.05.085