Fifty-two polymorphic microsatellite loci in the rust fungus, Cronartium quercuum f.sp. fusiforme

We report on 52 microsatellite markers for use in Cronartium quercuum f.sp. fusiforme. The markers were developed from di-, tri-, and tetranucleotide repeat-enriched genomic libraries. In 46 isolates collected from two natural populations in the southeastern USA, the number of alleles per locus rang...

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Bibliographic Details
Published in:Molecular ecology notes 2007-11, Vol.7 (6), p.1005-1008
Main Authors: BURDINE, C.S, KUBISIAK, T.L, JOHNSON, G.N, NELSON, C.D
Format: Article
Language:English
Subjects:
Cronartium quercuum
Cronartium quercuum f.sp. fusiforme
fusiform rust fungus
microsatellite
microsatellite repeats
polymorphism
simple sequence repeat
SSR marker
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Summary:We report on 52 microsatellite markers for use in Cronartium quercuum f.sp. fusiforme. The markers were developed from di-, tri-, and tetranucleotide repeat-enriched genomic libraries. In 46 isolates collected from two natural populations in the southeastern USA, the number of alleles per locus ranged from two to 20 (mean 6.94) with gene diversity values ranging from 0.043 to 0.933 (mean 0.537). The markers should prove highly useful for genetic 'fingerprinting' of single-spore isolates commonly used in host-pathogen gene interaction studies, as marker loci for linkage mapping studies, and for examining fine-scale population genetic structure in natural populations of the fungus.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2007.01756.x