Airway reconstruction using decellularized tracheal allografts in a porcine model

Purpose Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Methods Six weaned pigs (4-week-old domestic males) were transplanted with...

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Bibliographic Details
Published in:Pediatric surgery international 2017-10, Vol.33 (10), p.1065-1071
Main Authors: Ohno, Michinobu, Fuchimoto, Yasushi, Hsu, Huai-Che, Higuchi, Masataka, Komura, Makoto, Yamaoka, Tetsuji, Umezawa, Akihiro, Enosawa, Shin, Kuroda, Tatsuo
Format: Article
Language:English
Subjects:
Allografts - surgery
Animals
Male
Medicine
Medicine & Public Health
Models, Animal
Original Article
Pediatric Surgery
Pediatrics
Surgery
Swine
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds
Trachea - surgery
Weaning
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Summary:Purpose Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Methods Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. Results No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. Conclusion Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-017-4138-8