Potential effect of compounds isolated from Coffea arabica against UV-B induced skin damage by protecting fibroblast cells

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2017-09, Vol.174, p.323-332
• Main Authors: Cho, Yong-Hun, Bahuguna, Ashutosh, Kim, Han-Hyuk, Kim, Dong-in, Kim, Hyeon-Jeong, Yu, Jae-Myo, Jung, Hyun-Gug, Jang, Jae-Yoon, Kwak, Jae-Hoon, Park, Geun-Hye, Kwon, O-jun, Cho, Young Je, An, Jeong Yeon, Jo, Cheorun, Kang, Sun Chul, An, Bong-Jeun
• Format: Article
• Language: English
• Subjects: 3,4,5-Tricaffeoyl quinic; Aging; Aging (artificial); Animals; Beans; Catechol; Cell Line; Chlorogenic acid; Chromatography; Coffea - chemistry; Coffea arabica; Coffee; Collagen; Collagen Type I - metabolism; Column chromatography; Deoxyribonucleic acid; DNA; DNA damage; Elastin; Extracellular matrix; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - radiation effects; Gene Expression Regulation, Enzymologic - drug effects; Inhibition; Interstitial collagenase; Intracellular Space - drug effects; Intracellular Space - metabolism; Intracellular Space - radiation effects; Lymphocytes B; Magnetic induction; Matrix metalloproteinase; Matrix metalloproteinases; Matrix Metalloproteinases - metabolism; Mice; MMP-1, 3, 9; NMR; Nuclear magnetic resonance; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Procollagen; Proteins; Pyrocatechol; Quinic acid; Radiation damage; Radiation-Protective Agents - isolation & purification; Radiation-Protective Agents - pharmacology; Reactive oxygen species; Reactive Oxygen Species - metabolism; Side effects; Skin; Sun protection factor; Type-I procollagen; Ultraviolet radiation; Ultraviolet Rays - adverse effects; Xanthine oxidase; Xanthine Oxidase - metabolism
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2017.08.015

Ultraviolet (UV) radiation has adverse effects on extracellular matrix (ECM) proteins, leading to formation of wrinkles a hallmark of premature skin aging. The adverse effects of UV radiation are associated with induction of matrix metalloproteinases (MMPs) expression and degradation of collagen and elastin. The present study investigated anti-wrinkle effects of chlorogenic acid (CGA), pyrocatechol (PC) and 3,4,5-tricaffeoyl quinic acid (TCQ), isolated from beans of Coffea arabica, against UV-B stimulated mouse fibroblast cells (CCRF) by measuring expression levels of MMP-1, 3, 9, and type-I procollagen. The three compounds were isolated and purified from coffee grounds using column chromatography and structural examination was evaluated by nuclear magnetic resonance (NMR) analysis. Among the three isolated compounds, CGA effectively suppressed the expression of the MMP-1, 3, and 9 and increased synthesis of type-I procollagen as compared UV-B-stimulated CCRF cells. In addition, CGA dose-dependently inhibited intracellular reactive oxygen species (ROS) production in CCRF cells stimulated by UV radiation. Moreover, CGA displayed a good sun protection factor (SPF) and in vitro DNA damage protection together with inhibition of enzyme xanthine oxidase. The enzyme inhibitory kinetic behavior of CGA was determined by Lineweaver-Burk plot, displayed a mixed type enzyme inhibition with 260.3±4.5μM, Ki value. The results indicate that CGA has potential to be used as a preventive agent against premature skin aging induced by UV radiation. [Display omitted] •In vitro anti-wrinkle effects of chlorogenic acid (CGA), pyrocatechol (PC) and 3,4,5-tricaffeoyl quinic acid (TCQ)•CGA down-regulate MMP-1, 3, and 9 expressions•Type-I procollagen level up-regulated by CGA•CGA protected DNA damage induced by ROS production.