A reverse-hybridization test for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs

• Published in: Journal of microbiological methods 2017-12, Vol.143, p.13-16
• Main Authors: Marimón, José M., Morales, María, Gamen, Susana, Manrique, Ana, Ercibengoa, María, Cilla, Gustavo
• Format: Article
• Language: English
• Subjects: Capsular typing; Humans; Molecular Diagnostic Techniques - methods; Nucleic Acid Hybridization - methods; Pneumococcal Infections - diagnosis; Pneumococcal Infections - microbiology; Reverse-hybridization; Sensitivity and Specificity; Serogroup; Serotyping; Streptococcus pneumoniae; Streptococcus pneumoniae - classification; Streptococcus pneumoniae - genetics; Streptococcus pneumoniae - isolation & purification
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2017.08.010

The S. PneumoStrip test is a recently developed reverse hybridization strip-based commercial assay that allows for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs, according to their specific gene sequences. The test was validated with reference strains of 92 different pneumococcal serotypes and with a selection of 75 clinical isolates representing 55 serotypes, showing 100% sensitivity and specificity. The test was also applied to 64 pneumococcal invasive isolates (23 different serotypes) consecutively collected between June 2016 and March 2017, with 60 (93.8%) being serotyped. Four isolates belonging to serotypes 13, 29, and 35B (2 isolates), which are not included in the test, did not produce a hybridization signal with serotype specific probes. The identification of most serotypes causing invasive pneumococcal disease together with the simplicity of performance and results interpretation, and the use of routine laboratory equipment make this test very suitable for most clinical and research laboratories. •S. PneumoStrip is a reverse hybridization strip assay for pneumococcal serotyping.•It allows for the identification of 76 pneumococcal serotypes.•42 pneumococcal serotypes are identified individually and 34 in pairs.•60/64 (93.8%) pneumococcal invasive isolates collected in 2016–2017 were serotyped.