Short communication: A study of Lactobacillus isolates' adherence to and influence on membrane integrity of human Caco-2 cells

The selection criteria of ideal probiotic bacteria are complex and involve many factors. One key criterion is based on the ability of the probiotic bacteria to adhere to the epithelial lining of the gastrointestinal tract. The objective of this study was to evaluate and compare the adherence and inf...

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Bibliographic Details
Published in:Journal of dairy science 2017-10, Vol.100 (10), p.7891-7896
Main Authors: Jose, Neethu M., Bunt, Craig R., McDowell, Arlene, Chiu, Jasper Z.S., Hussain, Malik A.
Format: Article
Language:English
Subjects:
adherence
Animals
Bacterial Adhesion - physiology
Caco-2 Cells
Cattle
Cell Membrane - microbiology
Cell Membrane Structures
Escherichia coli
Escherichia coli - physiology
Humans
lactobacilli
Lactobacillus plantarum - physiology
Lactobacillus rhamnosus - physiology
permeability
Probiotics - isolation & purification
Rumen - microbiology
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Summary:The selection criteria of ideal probiotic bacteria are complex and involve many factors. One key criterion is based on the ability of the probiotic bacteria to adhere to the epithelial lining of the gastrointestinal tract. The objective of this study was to evaluate and compare the adherence and influence on membrane integrity of 2 selected lactobacilli isolates—Lactobacillus rhamnosus MI13 (dairy food origin) and L. plantarum RC2 (bovine rumen origin)—to Caco-2 cells in the presence and absence of Escherichia coli. The adhesion and influence on membrane integrity properties of the 2 Lactobacillus isolates were compared with Escherichia coli, a human commensal bacterium. From the adhesion studies, we concluded that the bovine rumen isolate exhibited better adherence to Caco-2 cells than the dairy food isolate. In contrast, the dairy food isolate better protected the Caco-2 monolayer from damage induced by ethanol.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2017-12912