Construction of an integrated gene regulatory network link to stress-related immune system in cattle

The immune system is an important biological system that is negatively impacted by stress. This study constructed an integrated regulatory network to enhance our understanding of the regulatory gene network used in the stress-related immune system. Module inference was used to construct modules of c...

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Bibliographic Details
Published in:Genetica 2017-10, Vol.145 (4-5), p.441-454
Main Authors: Behdani, Elham, Bakhtiarizadeh, Mohammad Reza
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
Cattle
Cattle Diseases - genetics
Cattle Diseases - immunology
Evolutionary Biology
Gene Regulatory Networks
Genes
Human Genetics
Immune response
Immune system
Immune System - physiology
Life Sciences
Male
Microbial Genetics and Genomics
Modules
Original Paper
Plant Genetics and Genomics
Promoter Regions, Genetic
Protein interaction
Ribonucleic acid
RNA
RNA, Messenger - metabolism
Stress, Physiological - genetics
Stress, Physiological - immunology
Stresses
Transcription factors
Transcription Factors - metabolism
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Summary:The immune system is an important biological system that is negatively impacted by stress. This study constructed an integrated regulatory network to enhance our understanding of the regulatory gene network used in the stress-related immune system. Module inference was used to construct modules of co-expressed genes with bovine leukocyte RNA-Seq data. Transcription factors (TFs) were then assigned to these modules using Lemon-Tree algorithms. In addition, the TFs assigned to each module were confirmed using the promoter analysis and protein–protein interactions data. Therefore, our integrated method identified three TFs which include one TF that is previously known to be involved in immune response (MYBL2) and two TFs (E2F8 and FOXS1) that had not been recognized previously and were identified for the first time in this study as novel regulatory candidates in immune response. This study provides valuable insights on the regulatory programs of genes involved in the stress-related immune system.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-017-9980-z