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Identification and characterization of the α- l-arabinofuranosidase B of Fusarium oxysporum f. sp. dianthi
The gene encoding α- l-Arabinofuranosidase B ( abfB) from the phytopathogenic fungus Fusarium oxysporum f. sp. dianthi ( Fod) was identified, cloned, sequenced and heterologously expressed. AbfB consists in an intronless open reading frame of 1500-bp coding for a protein of 499 aminoacid residues. T...
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| Published in: | Physiological and molecular plant pathology 2004-04, Vol.64 (4), p.201-208 |
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| Main Authors: | , , , , , , |
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| container_end_page | 208 |
| container_issue | 4 |
| container_start_page | 201 |
| container_title | Physiological and molecular plant pathology |
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| creator | Chacón-Martínez, Carlos A. Anzola, Juan M. Rojas, Andrés Hernández, Freddy Junca, Howard Ocampo, Walter Del Portillo, Patricia |
| description | The gene encoding α-
l-Arabinofuranosidase B (
abfB) from the phytopathogenic fungus
Fusarium oxysporum f. sp.
dianthi (
Fod) was identified, cloned, sequenced and heterologously expressed.
AbfB consists in an intronless open reading frame of 1500-bp coding for a protein of 499 aminoacid residues. The partially purified fusion protein has an apparent molecular mass of approximately 58
kDa and exhibits α-
l-arabinofuranosidase activity. The specific activity of the recombinant enzyme was 1.07 units mg
−1 protein. Optimal activity is attained at pH 4.0 and 50
°C. Interestingly, the
abfB gene is actively transcribed in carnation plants infected with
Fod. Its upregulation during the infection process suggests a possible role of this gene as virulence factor in the phytopathogenicity of this fungus. |
| doi_str_mv | 10.1016/j.pmpp.2004.08.005 |
| format | article |
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l-Arabinofuranosidase B (
abfB) from the phytopathogenic fungus
Fusarium oxysporum f. sp.
dianthi (
Fod) was identified, cloned, sequenced and heterologously expressed.
AbfB consists in an intronless open reading frame of 1500-bp coding for a protein of 499 aminoacid residues. The partially purified fusion protein has an apparent molecular mass of approximately 58
kDa and exhibits α-
l-arabinofuranosidase activity. The specific activity of the recombinant enzyme was 1.07 units mg
−1 protein. Optimal activity is attained at pH 4.0 and 50
°C. Interestingly, the
abfB gene is actively transcribed in carnation plants infected with
Fod. Its upregulation during the infection process suggests a possible role of this gene as virulence factor in the phytopathogenicity of this fungus.</description><identifier>ISSN: 0885-5765</identifier><identifier>EISSN: 1096-1178</identifier><identifier>DOI: 10.1016/j.pmpp.2004.08.005</identifier><identifier>CODEN: PMPPEZ</identifier><language>eng</language><publisher>London: Elsevier India Pvt Ltd</publisher><subject>alpha-N-arabinofuranosidase ; amino acid sequences ; Arabinase ; Biological and medical sciences ; Carnation ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; fungal proteins ; Fusarium oxysporum ; Fusarium oxysporum f. sp. dianthi ; Fusarium wilt ; gene expression regulation ; Hemicellulase ; molecular sequence data ; nucleotide sequences ; O-glycoside hydrolases ; open reading frames ; Phytopathogenic fungus ; Phytopathology. Animal pests. Plant and forest protection ; plant pathogenic fungi ; recombinant fusion proteins ; sequence analysis ; transcription (genetics) ; α- l-arabinofuranosidase</subject><ispartof>Physiological and molecular plant pathology, 2004-04, Vol.64 (4), p.201-208</ispartof><rights>2004 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-5119e8f1f40911a7d0de8cd9079fc9c996db220056cdbc2094af65fe8d4e2b033</citedby><cites>FETCH-LOGICAL-c331t-5119e8f1f40911a7d0de8cd9079fc9c996db220056cdbc2094af65fe8d4e2b033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16411567$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Chacón-Martínez, Carlos A.</creatorcontrib><creatorcontrib>Anzola, Juan M.</creatorcontrib><creatorcontrib>Rojas, Andrés</creatorcontrib><creatorcontrib>Hernández, Freddy</creatorcontrib><creatorcontrib>Junca, Howard</creatorcontrib><creatorcontrib>Ocampo, Walter</creatorcontrib><creatorcontrib>Del Portillo, Patricia</creatorcontrib><title>Identification and characterization of the α- l-arabinofuranosidase B of Fusarium oxysporum f. sp. dianthi</title><title>Physiological and molecular plant pathology</title><description>The gene encoding α-
l-Arabinofuranosidase B (
abfB) from the phytopathogenic fungus
Fusarium oxysporum f. sp.
dianthi (
Fod) was identified, cloned, sequenced and heterologously expressed.
AbfB consists in an intronless open reading frame of 1500-bp coding for a protein of 499 aminoacid residues. The partially purified fusion protein has an apparent molecular mass of approximately 58
kDa and exhibits α-
l-arabinofuranosidase activity. The specific activity of the recombinant enzyme was 1.07 units mg
−1 protein. Optimal activity is attained at pH 4.0 and 50
°C. Interestingly, the
abfB gene is actively transcribed in carnation plants infected with
Fod. Its upregulation during the infection process suggests a possible role of this gene as virulence factor in the phytopathogenicity of this fungus.</description><subject>alpha-N-arabinofuranosidase</subject><subject>amino acid sequences</subject><subject>Arabinase</subject><subject>Biological and medical sciences</subject><subject>Carnation</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>fungal proteins</subject><subject>Fusarium oxysporum</subject><subject>Fusarium oxysporum f. sp. dianthi</subject><subject>Fusarium wilt</subject><subject>gene expression regulation</subject><subject>Hemicellulase</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>O-glycoside hydrolases</subject><subject>open reading frames</subject><subject>Phytopathogenic fungus</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>plant pathogenic fungi</subject><subject>recombinant fusion proteins</subject><subject>sequence analysis</subject><subject>transcription (genetics)</subject><subject>α- l-arabinofuranosidase</subject><issn>0885-5765</issn><issn>1096-1178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQhy0EEkvhBbjgC9wSZpLYsSUuUNFSqRIH6Nny-g_rJRsHO6kob8WL8Ex1lErc4GTL_uanmfkIeYlQIyB_e6yn0zTVDUBXg6gB2COyQ5C8QuzFY7IDIVjFes6ekmc5HwFAdog78v3KunEOPhg9hzhSPVpqDjppM7sUfm2P0dP54Oif3xUdqvK3D2P0S9JjzMHq7OiHFblYsk5hOdH48y5PMZWbr2meamqDHudDeE6eeD1k9-LhPCM3Fx-_nn-qrj9fXp2_v65M2-JcMUTphEffgUTUvQXrhLESeumNNFJyu2_KpIwbuzdNGUR7zrwTtnPNHtr2jLzZcqcUfywuz-oUsnHDoEcXl6xQtojI-f_BvpOixTWx2UCTYs7JeTWlcNLpTiGoVYA6qlWAWgUoEKp0V4peP6TrbPTgy75MyH8reRHAeF-4VxvndVT6WyrMzZcGsC2OemjZSrzbCFe2dhtcUtkENxpnQ3JmVjaGfzVyD8ifprM</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Chacón-Martínez, Carlos A.</creator><creator>Anzola, Juan M.</creator><creator>Rojas, Andrés</creator><creator>Hernández, Freddy</creator><creator>Junca, Howard</creator><creator>Ocampo, Walter</creator><creator>Del Portillo, Patricia</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200404</creationdate><title>Identification and characterization of the α- l-arabinofuranosidase B of Fusarium oxysporum f. sp. dianthi</title><author>Chacón-Martínez, Carlos A. ; Anzola, Juan M. ; Rojas, Andrés ; Hernández, Freddy ; Junca, Howard ; Ocampo, Walter ; Del Portillo, Patricia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-5119e8f1f40911a7d0de8cd9079fc9c996db220056cdbc2094af65fe8d4e2b033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>alpha-N-arabinofuranosidase</topic><topic>amino acid sequences</topic><topic>Arabinase</topic><topic>Biological and medical sciences</topic><topic>Carnation</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>fungal proteins</topic><topic>Fusarium oxysporum</topic><topic>Fusarium oxysporum f. sp. dianthi</topic><topic>Fusarium wilt</topic><topic>gene expression regulation</topic><topic>Hemicellulase</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>O-glycoside hydrolases</topic><topic>open reading frames</topic><topic>Phytopathogenic fungus</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>plant pathogenic fungi</topic><topic>recombinant fusion proteins</topic><topic>sequence analysis</topic><topic>transcription (genetics)</topic><topic>α- l-arabinofuranosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chacón-Martínez, Carlos A.</creatorcontrib><creatorcontrib>Anzola, Juan M.</creatorcontrib><creatorcontrib>Rojas, Andrés</creatorcontrib><creatorcontrib>Hernández, Freddy</creatorcontrib><creatorcontrib>Junca, Howard</creatorcontrib><creatorcontrib>Ocampo, Walter</creatorcontrib><creatorcontrib>Del Portillo, Patricia</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Physiological and molecular plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chacón-Martínez, Carlos A.</au><au>Anzola, Juan M.</au><au>Rojas, Andrés</au><au>Hernández, Freddy</au><au>Junca, Howard</au><au>Ocampo, Walter</au><au>Del Portillo, Patricia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of the α- l-arabinofuranosidase B of Fusarium oxysporum f. sp. dianthi</atitle><jtitle>Physiological and molecular plant pathology</jtitle><date>2004-04</date><risdate>2004</risdate><volume>64</volume><issue>4</issue><spage>201</spage><epage>208</epage><pages>201-208</pages><issn>0885-5765</issn><eissn>1096-1178</eissn><coden>PMPPEZ</coden><abstract>The gene encoding α-
l-Arabinofuranosidase B (
abfB) from the phytopathogenic fungus
Fusarium oxysporum f. sp.
dianthi (
Fod) was identified, cloned, sequenced and heterologously expressed.
AbfB consists in an intronless open reading frame of 1500-bp coding for a protein of 499 aminoacid residues. The partially purified fusion protein has an apparent molecular mass of approximately 58
kDa and exhibits α-
l-arabinofuranosidase activity. The specific activity of the recombinant enzyme was 1.07 units mg
−1 protein. Optimal activity is attained at pH 4.0 and 50
°C. Interestingly, the
abfB gene is actively transcribed in carnation plants infected with
Fod. Its upregulation during the infection process suggests a possible role of this gene as virulence factor in the phytopathogenicity of this fungus.</abstract><cop>London</cop><pub>Elsevier India Pvt Ltd</pub><doi>10.1016/j.pmpp.2004.08.005</doi><tpages>8</tpages></addata></record> |
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| ispartof | Physiological and molecular plant pathology, 2004-04, Vol.64 (4), p.201-208 |
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| language | eng |
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| source | Elsevier |
| subjects | alpha-N-arabinofuranosidase amino acid sequences Arabinase Biological and medical sciences Carnation enzyme activity Fundamental and applied biological sciences. Psychology Fungal plant pathogens fungal proteins Fusarium oxysporum Fusarium oxysporum f. sp. dianthi Fusarium wilt gene expression regulation Hemicellulase molecular sequence data nucleotide sequences O-glycoside hydrolases open reading frames Phytopathogenic fungus Phytopathology. Animal pests. Plant and forest protection plant pathogenic fungi recombinant fusion proteins sequence analysis transcription (genetics) α- l-arabinofuranosidase |
| title | Identification and characterization of the α- l-arabinofuranosidase B of Fusarium oxysporum f. sp. dianthi |
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