Detection of intrathrombotic endothelial progenitor cells and its application to thrombus age estimation in a murine deep vein thrombosis model

Endothelial progenitor cells (EPCs), a newly identified cell type, are bone marrow-derived progenitor cells that co-express stem cell markers and Flk-1 (one of the receptors for vascular endothelial growth factor). In this study, double-color immunofluorescence analysis was performed using anti-CD34...

Full description

Saved in:
Bibliographic Details
Published in:International journal of legal medicine 2017-11, Vol.131 (6), p.1633-1638
Main Authors: Nosaka, Mizuho, Ishida, Yuko, Kimura, Akihiko, Yamamoto, Hiroki, Kato, Takashi, Kuninaka, Yumi, Shimada, Emi, Kondo, Toshikazu
Format: Article
Language:English
Subjects:
Age
Animals
Antibodies
Blood clots
Bone marrow
Cells (biology)
Chronology
Disease Models, Animal
Endothelial Progenitor Cells - pathology
Fluorescent Antibody Technique
Forensic Medicine
Forensic Pathology
Immunofluorescence
Medical Law
Medicine
Medicine & Public Health
Mice, Inbred BALB C
Microscopy, Fluorescence
Original Article
Receptors
Thrombosis
Time Factors
Venous Thrombosis - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Endothelial progenitor cells (EPCs), a newly identified cell type, are bone marrow-derived progenitor cells that co-express stem cell markers and Flk-1 (one of the receptors for vascular endothelial growth factor). In this study, double-color immunofluorescence analysis was performed using anti-CD34 and anti-Flk-1 antibodies in order to examine the time-dependent intrathrombotic appearance of EPCs, using the thrombi of DVT model mice with different thrombus ages (1–21 days). In thrombus cross-section specimens with an age of less than 3 days, CD34+/Flk-1+ EPCs were not detected. EPCs were initially observed in wounds aged 5 days, and their number was increased in thrombi with the advance of thrombus ages. The number of EPCs was the largest in the 10-day thrombus. Moreover, all 15 samples aged 7–14 days had an EPC number of more than 10, and, in 9 of them, the number of intrathrombotic EPCs was over 20. In contrast, in all thrombus samples aged 21 days, the number of intrathrombotic EPCs was less than 20. However, in three of them, the intrathrombotic EPC number was ≥ 10. These observations suggested that an intrathrombotic EPC number exceeding 20 would indicate a thrombus age of approximately 7–14 days.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-017-1668-5