Novel drug delivering conduit for peripheral nerve regeneration

Objective. This paper describes the design of a novel drug delivery apparatus integrated with a poly lactic-co-glycolic acid (PLGA) based nerve guide conduit for controlled local delivery of nerve growth factor (NGF) and application in peripheral nerve gap injury. Approach. An NGF dosage curve was a...

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Bibliographic Details
Published in:Journal of neural engineering 2017-12, Vol.14 (6), p.066011-066011
Main Authors: Labroo, Pratima, Shea, Jill, Edwards, Kyle, Ho, Scott, Davis, Brett, Sant, Himanshu, Goodwin, Isak, Gale, Bruce, Agarwal, Jay
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - administration & dosage
Chick Embryo
Dose-Response Relationship, Drug
drug delivery
Drug Delivery Systems - methods
Ganglia, Spinal - drug effects
Ganglia, Spinal - physiology
Infusion Pumps, Implantable
Lactic Acid - administration & dosage
Male
nerve conduit
Nerve Growth Factor - administration & dosage
Nerve Regeneration - drug effects
Nerve Regeneration - physiology
neurotrophic factors
peripheral nerve injury
Polyglycolic Acid - administration & dosage
Polylactic Acid-Polyglycolic Acid Copolymer
Rats
Rats, Sprague-Dawley
reservoir
Sciatic Neuropathy - drug therapy
Sciatic Neuropathy - pathology
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Summary:Objective. This paper describes the design of a novel drug delivery apparatus integrated with a poly lactic-co-glycolic acid (PLGA) based nerve guide conduit for controlled local delivery of nerve growth factor (NGF) and application in peripheral nerve gap injury. Approach. An NGF dosage curve was acquired to determine the minimum in vitro concentration for optimal neurite outgrowth of dorsal root ganglion (DRG) cells; PLGA based drug delivery devices were then designed and tested in vitro and in vivo across 15 mm rat sciatic nerve gap injury model. Main results. The drug delivery nerve guide was able to release NGF for 28 d at concentrations (0.1-10 ng ml−1) that were shown to enhance DRG neurite growth. Furthermore, the released NGF was bioactive and able to enhance DRG neurite growth. Following these tests, optimized NGF-releasing nerve conduits were implanted across 15 mm sciatic nerve gaps in a rat model, where they demonstrated significant myelination and muscle innervation in vivo as compared to empty nerve conduits (p  
ISSN:1741-2560
1741-2552
DOI:10.1088/1741-2552/aa867d