Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone

[Display omitted] •HPLC-CL method was developed for PQQ analysis using the redox cycle of quinone.•The method was ultrasensitive to PQQ with LOD of 1.08nmol/L plasma (0.27nM).•A novel solid phase extraction (SPE) method was developed for PQQ from human plasma.•The developed SPE method was simple, fa...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2017-10, Vol.145, p.814-820
Main Authors: Fukuda, Mizuho, El-Maghrabey, Mahmoud H., Kishikawa, Naoya, Ikemoto, Kazuto, Kuroda, Naotaka
Format: Article
Language:English
Subjects:
Chemiluminescence
Chromatography, High Pressure Liquid
HPLC
Human plasma
Humans
Luminescence
Oxidation-Reduction
PQQ Cofactor
Pyrroloquinoline quinone
Quinones - blood
Redox cycle
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Summary:[Display omitted] •HPLC-CL method was developed for PQQ analysis using the redox cycle of quinone.•The method was ultrasensitive to PQQ with LOD of 1.08nmol/L plasma (0.27nM).•A novel solid phase extraction (SPE) method was developed for PQQ from human plasma.•The developed SPE method was simple, fast, and efficient (recovery >95%).•The method was applied to monitor PQQ in human plasma after administration of PQQ. A fast, accurate, and ultrasensitive high-performance liquid chromatography method with chemiluminescence detection (HPLC-CL) was optimized and validated for the determination of pyrroloquinoline quinone (PQQ) concentration in human plasma following solid-phase extraction (SPE). This method is based on the redox cycle of the reaction between PQQ and dithiothreitol, which generates reactive oxygen species that can be detected using luminol as a CL probe. The isocratic HPLC system comprised an ODS column and 4.0mM tetra-n-butylammonium bromide in Tris-HNO3 buffer (pH 8.8; 50mM)-acetonitrile (7:3, v/v) as mobile phase. A novel, rapid, and simple SPE method was also developed providing excellent %recovery (≥95.2%) for PQQ from human plasma samples. The proposed method was linear over the range of 4.0–400nmol/L plasma of PQQ with a lower detection limit (S/N=3) of 1.08 nmol/L plasma (0.27nM). The method was successfully implemented to determine PQQ concentration in the plasma of healthy individuals after administration of PQQ supplements.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2017.08.008