Loading…

First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells

Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety...

Full description

Saved in:
Bibliographic Details
Published in:Genetics and molecular research 2017-08, Vol.16 (3), p.1
Main Authors: Marques, E S, Tsuboy, M S F, Carvalho, J C T, Rosa, P C P, Perazzo, F F, Gaivão, I O M, Maistro, E L
Format: Article
Language:English
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 3
container_start_page 1
container_title Genetics and molecular research
container_volume 16
creator Marques, E S
Tsuboy, M S F
Carvalho, J C T
Rosa, P C P
Perazzo, F F
Gaivão, I O M
Maistro, E L
description Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 µg/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a]pyrene, used as DNA-damaging agents.
doi_str_mv 10.4238/gmr16039700
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1931711131</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1931711131</sourcerecordid><originalsourceid>FETCH-LOGICAL-p239t-2cbaf0a06ba361dfd2e673336e0308ab6269927c8f2f642f3ecbfb48b3efb3a93</originalsourceid><addsrcrecordid>eNpdkMtKAzEUhoMgtlZX7iXgpoKjSU6bSZZSWhUKbnRdMjMndcrczAXtE7nyKfpijmhduDic28fPz0_IGWfXEwHqZl07LhnolLEDMuQylclUKjYgx95vGBPTiWJHZCCUElppGJK3Rel8oPk2tKF9L_MrusZmP5qm6CuUfydqvEfva2wCbS2dx4CuQ9pW6EyOhloXy_5TVnRsdh9m93lJy4bmsQrRYUFfYm36FavKn5BDayqPp799RJ4X86fZfbJ8vHuY3S6TToAOicgzY5lhMjMgeWELgTIFAIkMmDKZFFJrkebKCisnwgLmmc0mKgO0GRgNIzL-0e1c-xrRh1Vd-m8HpsE2-hXXwFPOOfAevfiHbtromt5dT2lQfWBK9dT5LxWzGotV58rauO1qHyl8AUVueNc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993898988</pqid></control><display><type>article</type><title>First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells</title><source>Alma/SFX Local Collection</source><creator>Marques, E S ; Tsuboy, M S F ; Carvalho, J C T ; Rosa, P C P ; Perazzo, F F ; Gaivão, I O M ; Maistro, E L</creator><creatorcontrib>Marques, E S ; Tsuboy, M S F ; Carvalho, J C T ; Rosa, P C P ; Perazzo, F F ; Gaivão, I O M ; Maistro, E L</creatorcontrib><description>Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 µg/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a]pyrene, used as DNA-damaging agents.</description><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/gmr16039700</identifier><identifier>PMID: 28829893</identifier><language>eng</language><publisher>Brazil: Fundacao de Pesquisas Cientificas de Ribeirao Preto</publisher><subject>Benzo(a)pyrene ; Cells, Cultured ; Chemoprotection ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA Damage ; Economic importance ; Euterpe - chemistry ; Euterpe oleracea ; Fruit - chemistry ; Genotoxicity ; Hep G2 Cells ; Hepatoma ; Humans ; Lymphocytes ; Lymphocytes - drug effects ; Methyl methanesulfonate ; Plant Oils - pharmacology ; Plant Oils - toxicity</subject><ispartof>Genetics and molecular research, 2017-08, Vol.16 (3), p.1</ispartof><rights>Copyright Fundacao de Pesquisas Cientificas de Ribeirao Preto 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28829893$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marques, E S</creatorcontrib><creatorcontrib>Tsuboy, M S F</creatorcontrib><creatorcontrib>Carvalho, J C T</creatorcontrib><creatorcontrib>Rosa, P C P</creatorcontrib><creatorcontrib>Perazzo, F F</creatorcontrib><creatorcontrib>Gaivão, I O M</creatorcontrib><creatorcontrib>Maistro, E L</creatorcontrib><title>First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 µg/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a]pyrene, used as DNA-damaging agents.</description><subject>Benzo(a)pyrene</subject><subject>Cells, Cultured</subject><subject>Chemoprotection</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>Economic importance</subject><subject>Euterpe - chemistry</subject><subject>Euterpe oleracea</subject><subject>Fruit - chemistry</subject><subject>Genotoxicity</subject><subject>Hep G2 Cells</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Lymphocytes</subject><subject>Lymphocytes - drug effects</subject><subject>Methyl methanesulfonate</subject><subject>Plant Oils - pharmacology</subject><subject>Plant Oils - toxicity</subject><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpdkMtKAzEUhoMgtlZX7iXgpoKjSU6bSZZSWhUKbnRdMjMndcrczAXtE7nyKfpijmhduDic28fPz0_IGWfXEwHqZl07LhnolLEDMuQylclUKjYgx95vGBPTiWJHZCCUElppGJK3Rel8oPk2tKF9L_MrusZmP5qm6CuUfydqvEfva2wCbS2dx4CuQ9pW6EyOhloXy_5TVnRsdh9m93lJy4bmsQrRYUFfYm36FavKn5BDayqPp799RJ4X86fZfbJ8vHuY3S6TToAOicgzY5lhMjMgeWELgTIFAIkMmDKZFFJrkebKCisnwgLmmc0mKgO0GRgNIzL-0e1c-xrRh1Vd-m8HpsE2-hXXwFPOOfAevfiHbtromt5dT2lQfWBK9dT5LxWzGotV58rauO1qHyl8AUVueNc</recordid><startdate>20170817</startdate><enddate>20170817</enddate><creator>Marques, E S</creator><creator>Tsuboy, M S F</creator><creator>Carvalho, J C T</creator><creator>Rosa, P C P</creator><creator>Perazzo, F F</creator><creator>Gaivão, I O M</creator><creator>Maistro, E L</creator><general>Fundacao de Pesquisas Cientificas de Ribeirao Preto</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170817</creationdate><title>First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells</title><author>Marques, E S ; Tsuboy, M S F ; Carvalho, J C T ; Rosa, P C P ; Perazzo, F F ; Gaivão, I O M ; Maistro, E L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-2cbaf0a06ba361dfd2e673336e0308ab6269927c8f2f642f3ecbfb48b3efb3a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Benzo(a)pyrene</topic><topic>Cells, Cultured</topic><topic>Chemoprotection</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Economic importance</topic><topic>Euterpe - chemistry</topic><topic>Euterpe oleracea</topic><topic>Fruit - chemistry</topic><topic>Genotoxicity</topic><topic>Hep G2 Cells</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Lymphocytes</topic><topic>Lymphocytes - drug effects</topic><topic>Methyl methanesulfonate</topic><topic>Plant Oils - pharmacology</topic><topic>Plant Oils - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marques, E S</creatorcontrib><creatorcontrib>Tsuboy, M S F</creatorcontrib><creatorcontrib>Carvalho, J C T</creatorcontrib><creatorcontrib>Rosa, P C P</creatorcontrib><creatorcontrib>Perazzo, F F</creatorcontrib><creatorcontrib>Gaivão, I O M</creatorcontrib><creatorcontrib>Maistro, E L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marques, E S</au><au>Tsuboy, M S F</au><au>Carvalho, J C T</au><au>Rosa, P C P</au><au>Perazzo, F F</au><au>Gaivão, I O M</au><au>Maistro, E L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2017-08-17</date><risdate>2017</risdate><volume>16</volume><issue>3</issue><spage>1</spage><pages>1-</pages><eissn>1676-5680</eissn><abstract>Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 µg/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a]pyrene, used as DNA-damaging agents.</abstract><cop>Brazil</cop><pub>Fundacao de Pesquisas Cientificas de Ribeirao Preto</pub><pmid>28829893</pmid><doi>10.4238/gmr16039700</doi></addata></record>
fulltext fulltext
identifier EISSN: 1676-5680
ispartof Genetics and molecular research, 2017-08, Vol.16 (3), p.1
issn 1676-5680
language eng
recordid cdi_proquest_miscellaneous_1931711131
source Alma/SFX Local Collection
subjects Benzo(a)pyrene
Cells, Cultured
Chemoprotection
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA Damage
Economic importance
Euterpe - chemistry
Euterpe oleracea
Fruit - chemistry
Genotoxicity
Hep G2 Cells
Hepatoma
Humans
Lymphocytes
Lymphocytes - drug effects
Methyl methanesulfonate
Plant Oils - pharmacology
Plant Oils - toxicity
title First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A50%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=First%20cytotoxic,%20genotoxic,%20and%20antigenotoxic%20assessment%20of%20Euterpe%20oleracea%20fruit%20oil%20(a%C3%A7a%C3%AD)%20in%20cultured%20human%20cells&rft.jtitle=Genetics%20and%20molecular%20research&rft.au=Marques,%20E%20S&rft.date=2017-08-17&rft.volume=16&rft.issue=3&rft.spage=1&rft.pages=1-&rft.eissn=1676-5680&rft_id=info:doi/10.4238/gmr16039700&rft_dat=%3Cproquest_pubme%3E1931711131%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p239t-2cbaf0a06ba361dfd2e673336e0308ab6269927c8f2f642f3ecbfb48b3efb3a93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1993898988&rft_id=info:pmid/28829893&rfr_iscdi=true