Analysis of the peptides detected in atopic dermatitis and various inflammatory diseases patients-derived sera

• Published in: International journal of biological macromolecules 2018-01, Vol.106, p.1052-1061
• Main Authors: Yin, Shang-Jun, Cho, Ick-Hyun, Yang, Hee Seung, Park, Yong-Doo, Yang, Jun-Mo
• Format: Article
• Language: English
• Subjects: Atopic dermatitis; Biomarkers - blood; Dermatitis, Atopic - blood; Dermatitis, Atopic - genetics; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation - genetics; Humans; Inflammation - blood; Inflammation - pathology; Peptides; Peptides - blood; Peptides - genetics; Proteome - genetics; Proteomics; Serum proteomics
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.08.109

Serum proteomics has been applied for the discovery and analysis of biomarkers related to human disease. Serum is an optimal source to identify proteins derived from diseased-tissue compartments. We recently established an integrative method to analyze highly basic proteins that remain unresolved by the general 2D-PAGE method. In this follow-up study, we successfully detected several disease-associated proteins from sera samples obtained from patients with atopic dermatitis (AD). After proteomic analyses, target proteins were validated from AD patient-derived sera using ELISA or Western blotting methods We detected zinc finger CCHC domain containing 10 (ZCCHC10), peptidoglycan recognition protein L (PGRP-L), kininogen, α-1-antitrypsin, and hornerin proteins that are dysregulated in AD patient sera samples, which suggest effective approaches to methodologically analyze the serum proteome. Thus, the integrated proteomic method approach described here could be applicable for the detection of proteins associated with other human diseases. Our present study provides new insights into optimized serum proteomic techniques to understand systemic events of AD.