Background Nutrients Affect the Biotransformation of Tetracycline by Stenotrophomonas maltophilia as Revealed by Genomics and Proteomics

Certain bacteria are resistant to antibiotics and can even transform antibiotics in the environment. It is unclear how the molecular mechanisms underlying the resistance and biotransformation processes vary under different environmental conditions. The objective of this study is to investigate the m...

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Bibliographic Details
Published in:Environmental science & technology 2017-09, Vol.51 (18), p.10476-10484
Main Authors: Leng, Yifei, Bao, Jianguo, Song, Dandan, Li, Jing, Ye, Mao, Li, Xu
Format: Article
Language:English
Subjects:
Analysis
Anti-Bacterial Agents
Antibiotics
Bacteria
Bacterial Proteins
Binding
Biotransformation
Citric acid
Drug resistance
Environment
Environmental conditions
Genomic analysis
Genomics
Guanine
Hypoxanthine
Molecular modelling
Monooxygenase
Nodulation
Nutrients
Oxidation
Peptones
Peroxidase
Peroxiredoxin
Phosphoribosyltransferase
Protein transport
Proteins
Proteomics
Stenotrophomonas maltophilia
Stenotrophomonas maltophilia - genetics
Stenotrophomonas maltophilia - physiology
Superoxide dismutase
Tetracycline - metabolism
Tetracycline Resistance - genetics
Water Pollutants, Chemical - metabolism
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Summary:Certain bacteria are resistant to antibiotics and can even transform antibiotics in the environment. It is unclear how the molecular mechanisms underlying the resistance and biotransformation processes vary under different environmental conditions. The objective of this study is to investigate the molecular mechanisms of tetracycline resistance and biotransformation by Stenotrophomonas maltophilia strain DT1 under various background nutrient conditions. Strain DT1 was exposed to tetracycline for 7 days with four background nutrient conditions: no background (NB), peptone (P), peptone plus citrate (PC), and peptone plus glucose (PG). The biotransformation rate follows the order of PC > P > PG > NB ≈ 0. Genomic analysis showed that strain DT1 contained tet(X1), a gene encoding an FAD-binding monooxygenase, and eight peroxidase genes that could be relevant to tetracycline biotransformation. Quantitative proteomic analyses revealed that nodulation protein transported tetracycline outside of cells; hypoxanthine–guanine phosphoribosyltransferase facilitated the activation of the ribosomal protection proteins to prevent the binding of tetracycline to the ribosome and superoxide dismutase and peroxiredoxin-modified tetracycline molecules. Comparing different nutrient conditions showed that the biotransformation rates of tetracycline were positively correlated with the expression levels of superoxide dismutase.
ISSN:0013-936X
1520-5851
DOI:10.1021/acs.est.7b02579