Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED

• Published in: Archives of oral biology 2017-11, Vol.83, p.258-264
• Main Authors: Almeida, Leopoldina de Fátima Dantas de, Basso, Fernanda Gonçalves, Turrioni, Ana Paula Silveira, de-Souza-Costa, Carlos Alberto, Hebling, Josimeri
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - metabolism; Cell Line; Cell Proliferation - radiation effects; Cell Survival - radiation effects; Cells, Cultured; Collagen - metabolism; dental pulp; dentin; Dentistry; Humans; Light; light emitting diodes; Molar; odontoblasts; Odontoblasts - cytology; Odontoblasts - metabolism; Odontoblasts - radiation effects; phototerapy
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2017.08.004

•The odontoblast like cells MDPC-23 exposed to red LED (630nm) and blue LED (540) were evaluated with regards to celular metabolismo.•We evaluated two different power densities (40 e 80mW/cm2) according to waveleght.•Red LED at the energy dose of 4J/cm2 increased cell viability.•Blue LED was not capable of photobiostimulating cell proliferation. The present study evaluated the trans-dentinal effect of light emitting diodes (LEDs) irradiation on the metabolism of odontoblast-like cells. Seventy-two dentin discs (0.2mm thick) were obtained from human molar teeth. MDPC-23 cells (20,000 cells/disc) were seeded on the pulpal side of the discs using DMEM, supplemented with 10% fetal bovine serum (FBS). After 12h, the culture medium was replaced with DMEM containing 0.5% FBS. After additional 12h, blue (455±10nm) or red (630±10nm) LEDs were used at irradiances of 80 and 40mW/cm2, respectively, to irradiate the occlusal side of the discs. The energy doses were fixed at 2 or 4J/cm2. Cell viability, alkaline phosphatase activity (ALP), total protein production and collagen synthesis were evaluated 72h after irradiation. Data were submitted to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Red light promoted proliferative effects at the energy dose of 4J/cm2. Conversely, cell cultures irradiated with 2J/cm2 emitted by the blue light showed reduced viability. ALP production was stimulated by red light in comparison with blue light at 4J/cm2. Total protein production was reduced after exposure to blue light at 4J/cm2, while no effect was observed on collagen production. Irradiation with red LED at 4J/cm2 bio-stimulated the viability of odontoblast-like cells, whilst blue light had unfavorable effects on the cellular metabolism.