Low efficiency of pseudotestcross mapping design was consistent with limited genetic diversity and low heterozygosity in hoop pine (Araucaria cunninghamii, Araucariaceae)

Linkage maps were prepared for two Araucaria cunninghamii individuals (coded H15 and Gil24) using the pseudotestcross strategy in a wide interprovenance cross. The maternal map for individual H15 contains 14 linkage groups (haploid chromosome number=13), comprising 51 amplified fragment length polym...

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Bibliographic Details
Published in:Tree genetics & genomes 2005-11, Vol.1 (3), p.124-134
Main Authors: Scott, L. J, Shepherd, M. J, Nikles, D. G, Henry, R. J
Format: Article
Language:English
Subjects:
amplified fragment length polymorphism
Araucaria cunninghamii
Araucariaceae
chromosome mapping
conifers
genetic variation
heterozygosity
microsatellite repeats
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Summary:Linkage maps were prepared for two Araucaria cunninghamii individuals (coded H15 and Gil24) using the pseudotestcross strategy in a wide interprovenance cross. The maternal map for individual H15 contains 14 linkage groups (haploid chromosome number=13), comprising 51 amplified fragment length polymorphisms (AFLP) and 1 microsatellite; 17 markers remain unlinked. The map covered 1,290 cM [Kosambi (K)], representing 89% of the estimated genome size. The paternal map for individual Gil24 was shorter, 633 cM (K), consisted of eight linkage groups, with an average interval of 19.8 cM (K). The difference in map lengths was due to the larger number of informative markers for maternal parent (52 loci compared with 25 loci in the paternal parent). There was no significant difference in map lengths once maps were corrected for different numbers of loci. Overall, the number of segregating markers identified was surprisingly low for a wide interprovenance cross in an outcrossing tree species. For AFLP, a low average of 2.2 segregating markers per primer combination was obtained, and only 4 out of 29 microsatellite loci were informative in the cross. This low level of marker variation appears to be the result of low levels of heterozygosity in the parents and low levels of genetic divergence within A. cunninghamii. This result was consistent with other recent molecular studies of A. cunninghamii that indicate that the species may have low genetic diversity and possibly experiences localised inbreeding.
ISSN:1614-2942
1614-2950
DOI:10.1007/s11295-005-0022-0