The Influence of Proinflammatory Factors on the Neuroprotective Efficiency of Multipotent Mesenchymal Stromal Cells in Traumatic Brain Injury

We studied the neuroprotective potential of multipotent mesenchymal stromal cells in traumatic brain injury and the effect of inflammatory preconditioning on neuroprotective properties of stem cells under in vitro conditions. To this end, the effects of cell incubation with LPS or their co-culturing...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2017-08, Vol.163 (4), p.528-534
Main Authors: Danilina, T. I., Silachev, D. N., Pevzner, I. B., Gulyaev, M. V., Pirogov, Yu. A., Zorova, L. D., Plotnikov, E. Yu, Sukhikh, G. T., Zorov, D. B.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brain damage
Brain injuries
Brain Injuries - metabolism
Brain Injuries - pathology
Cell Biology
Cells, Cultured
Cytokines
Cytokines - metabolism
Inflammation
Inflammation - metabolism
Interleukin-1alpha - metabolism
Interleukin-6 - metabolism
Internal Medicine
Laboratory Medicine
Magnetic Resonance Imaging
Male
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - metabolism
Mesenchymal Stromal Cells - metabolism
Pathology
Rats
Stem cells
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Tumor Necrosis Factor-alpha - metabolism
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Summary:We studied the neuroprotective potential of multipotent mesenchymal stromal cells in traumatic brain injury and the effect of inflammatory preconditioning on neuroprotective properties of stem cells under in vitro conditions. To this end, the effects of cell incubation with LPS or their co-culturing with leukocytes on production of cytokines IL-1α, IL-6, TNFα, and MMP-2 and MMP-9 by these cells were evaluated. Culturing under conditions simulating inflammation increased the production of all these factors by multipotent mesenchymal stromal cells. However, acquisition of the inflammatory phenotype by stromal cells did not reduce their therapeutic effectiveness in traumatic brain injury. Moreover, in some variants of inflammatory preconditioning, multipotent mesenchymal stromal cells exhibited more pronounced neuroprotective properties reducing the volume of brain lesion and promoting recovery of neurological functions after traumatic brain injury.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-017-3844-5