Effect of different oxygen tension on the cytoskeleton remodeling of goat temporomandibular joint disc cells

Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O₂) and hypoxia (2%, 4%, and 8% O₂). Toluidine blue, picrosirius red, and type Ⅰ collagen...

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Published in:Hua xi kou qiang yi xue za zhi 2017-08, Vol.35 (4), p.362-367
Main Authors: Xiaolan, He, Guangjie, Bao, Linglu, Sun, Xue, Zhang, Shanying, Bao, Hong, Kang
Format: Article
Language:Chinese
Subjects:
Actins
Animals
Cell Line
Cells, Cultured
Collagen Type I
Cytoskeleton
Dentistry
Fibroblasts
Goats
Humans
Hypoxia
Oxygen
RNA, Messenger
Temporomandibular Joint Disc
Vimentin
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Summary:Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O₂) and hypoxia (2%, 4%, and 8% O₂). Toluidine blue, picrosirius red, and type Ⅰ collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse transcription-polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O₂(P
ISSN:1000-1182
DOI:10.7518/hxkq.2017.04.004