Effect of different oxygen tension on the cytoskeleton remodeling of goat temporomandibular joint disc cells

Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O₂) and hypoxia (2%, 4%, and 8% O₂). Toluidine blue, picrosirius red, and type Ⅰ collagen...

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Published in:Hua xi kou qiang yi xue za zhi 2017-08, Vol.35 (4), p.362-367
Main Authors: Xiaolan, He, Guangjie, Bao, Linglu, Sun, Xue, Zhang, Shanying, Bao, Hong, Kang
Format: Article
Language:Chinese
Subjects:
Actins
Animals
Cell Line
Cells, Cultured
Collagen Type I
Cytoskeleton
Dentistry
Fibroblasts
Goats
Humans
Hypoxia
Oxygen
RNA, Messenger
Temporomandibular Joint Disc
Vimentin
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container_title Hua xi kou qiang yi xue za zhi
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creator Xiaolan, He
Guangjie, Bao
Linglu, Sun
Xue, Zhang
Shanying, Bao
Hong, Kang
description Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O₂) and hypoxia (2%, 4%, and 8% O₂). Toluidine blue, picrosirius red, and type Ⅰ collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse transcription-polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O₂(P
doi_str_mv 10.7518/hxkq.2017.04.004
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Methods Goat TMJ disc cells were cultured under normoxia (21% O₂) and hypoxia (2%, 4%, and 8% O₂). Toluidine blue, picrosirius red, and type Ⅰ collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse transcription-polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O₂(P&lt;0.05), whereas that of tubulin was highest at 2% O₂ (P&lt;0.05). No significant difference among the other groups was observed (P&gt;0.05). Actin mRNA levels were considerably decreased at 2% O₂ and 4% O₂ in</description><identifier>ISSN: 1000-1182</identifier><identifier>DOI: 10.7518/hxkq.2017.04.004</identifier><identifier>PMID: 28853500</identifier><language>chi</language><publisher>China</publisher><subject>Actins ; Animals ; Cell Line ; Cells, Cultured ; Collagen Type I ; Cytoskeleton ; Dentistry ; Fibroblasts ; Goats ; Humans ; Hypoxia ; Oxygen ; RNA, Messenger ; Temporomandibular Joint Disc ; Vimentin</subject><ispartof>Hua xi kou qiang yi xue za zhi, 2017-08, Vol.35 (4), p.362-367</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28853500$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiaolan, He</creatorcontrib><creatorcontrib>Guangjie, Bao</creatorcontrib><creatorcontrib>Linglu, Sun</creatorcontrib><creatorcontrib>Xue, Zhang</creatorcontrib><creatorcontrib>Shanying, Bao</creatorcontrib><creatorcontrib>Hong, Kang</creatorcontrib><title>Effect of different oxygen tension on the cytoskeleton remodeling of goat temporomandibular joint disc cells</title><title>Hua xi kou qiang yi xue za zhi</title><addtitle>Hua Xi Kou Qiang Yi Xue Za Zhi</addtitle><description>Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. 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Methods Goat TMJ disc cells were cultured under normoxia (21% O₂) and hypoxia (2%, 4%, and 8% O₂). Toluidine blue, picrosirius red, and type Ⅰ collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse transcription-polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O₂(P&lt;0.05), whereas that of tubulin was highest at 2% O₂ (P&lt;0.05). No significant difference among the other groups was observed (P&gt;0.05). 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subjects Actins
Animals
Cell Line
Cells, Cultured
Collagen Type I
Cytoskeleton
Dentistry
Fibroblasts
Goats
Humans
Hypoxia
Oxygen
RNA, Messenger
Temporomandibular Joint Disc
Vimentin
title Effect of different oxygen tension on the cytoskeleton remodeling of goat temporomandibular joint disc cells
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