Observations of Forsythia koreana methanol extract on mast cell-mediated allergic reactions in experimental models

• Published in: In vitro cellular & developmental biology. Animal 2007-07, Vol.43 (7), p.215-221
• Main Authors: Choi, In-Young, Moon, Phil-Dong, Koo, Hyun-Na, Myung, Noh-Yil, Kim, Su-Jin, Lee, Ji-Hyun, Han, Se-Hee, Moon, Goo, Seo, Sung-Yum, Sung, Hyun-Jea, Park, Rae-Kil, Jeong, Hyun-Ja, Um, Jae-Young, Kim, Hyung-Min, Hong, Seung-Heon
• Format: Article
• Language: English
• Subjects: Allergies; Anaphylaxis; Anaphylaxis - chemically induced; Anaphylaxis - immunology; Animals; Anti-allergic inflammation; Cell and Tissue Models; Cell Line; Compound48/80; Cytokines; Forsythia; Forsythia - chemistry; Forsythia - immunology; Histamine; Histamine Release; Histamines; HMC-1 cells; Humans; Immunoglobulin E - immunology; Male; Mast cells; Mast Cells - cytology; Mast Cells - immunology; Methanol - chemistry; Mice; Mortality; p-Methoxy-N-methylphenethylamine - immunology; Passive cutaneous anaphylaxis; Passive Cutaneous Anaphylaxis - immunology; Plant Extracts - chemistry; Plant Extracts - immunology; Rats; Rats, Wistar; Secretion; Viability
• ISSN: 1071-2690; 1543-706X
• DOI: 10.1007/s11626-007-9040-6

To explore effects of Forsythia koreana methanol extract (FKME) on mast cell-mediated allergic and inflammatory properties, the effect of FKME was evaluated on compound 48/80-induced systemic anaphylaxis, ear swelling, and anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous anaphylaxis (PCA). In addition, the effect of FKME was investigated on the histamine release from rat peritoneal mast cells (RPMCs) stimulated by compound 48/80, which promotes histamine release. The human mast cell line HMC-1 was stimulated by phorbol 12-myristate 13-acetate plus calcium ionophore A23187. Activated HMC-1 can produce several proinflammatory and chemotactic cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-8. Cytokine levels in the culture supernatant were measured by an enzyme-linked immunosorbent assay. Cytotoxicity by FKME was determined by a 3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium bromide (MTT) assay. FKME inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in mice. When 1 g/kg FKME was pretreated or posttreated with mice, compound 48/80-induced mice morality was 50 and 66.7%, respectively. One gram per kilogram of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 by 29.75%. A PCA reaction was inhibited by 17.9%. In an in vitro model, FKME (1 mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-α, IL-6, and IL-8 production from HMC-1 cells by 71.16% (P