The effect of dental bleaching on pulpal tissue response in a diabetic animal model: a study of immunoregulatory cytokines

• Published in: International endodontic journal 2018-03, Vol.51 (3), p.347-356
• Main Authors: Ferreira, L. L., Gomes‐Filho, J. E., Benetti, F., Carminatti, M., Ervolino, E., Briso, A. L. F., Cintra, L. T. A.
• Format: Article
• Language: English
• Subjects: Bleaching; Citric acid; Cytokines; dental bleaching; Dental pulp; Dentistry; Diabetes; Diabetes mellitus; Endodontics; Hydrogen peroxide; Immunohistochemistry; Immunoregulation; Inflammation; inflammatory response; Interleukin 17; Interleukin 6; Maxilla; Metastases; Molars; proinflammatory cytokines; Rodents; Teeth; Tumor necrosis factor; Tumor necrosis factor-TNF; Tumors
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.12852

Aim To evaluate the influence of tooth bleaching on immunoregulatory cytokines production (IL‐6, Tumour necrosis factor (TNF)‐α and IL‐17) in the pulp tissue of normoglycaemic and diabetic rats. Methodology Twenty‐eight rats were divided into normoglycaemic and diabetic rats (n = 14). Diabetes mellitus (DM) was induced with a single dose of alloxan diluted in citrate buffer via intramuscular injection. After DM confirmation, all rats were sedated and tooth bleaching was performed using 35% hydrogen peroxide on the right maxillary molars for 30 min. Left molars were used as controls. Bleaching resulted in four hemimaxillae groups: normoglycaemic (N), N‐bleached (NBle), diabetic (D) and D‐bleached (DBle). After 2 and 30 days, rats were euthanized and hemimaxillae processed for analysis by haematoxylin and eosin and immunohistochemistry. Results within and between animals were submitted to Wilcoxon signed‐rank and Mann–Whitney tests (P