Proteomic analysis of silica hybrid sol-gel coatings: a potential tool for predicting the biocompatibility of implants in vivo

The interactions of implanted biomaterials with the host organism determine the success or failure of an implantation. Normally, their biocompatibility is assessed using in vitro tests. Unfortunately, in vitro and in vivo results are not always concordant; new, effective methods of biomaterial chara...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2017-09, Vol.33 (8), p.676-689
Main Authors: Romero-Gavilan, F., Sánchez-Pérez, A. M., Araújo-Gomes, N., Azkargorta, M., Iloro, I., Elortza, F., Gurruchaga, M., Goñi, I., Suay, J.
Format: Article
Language:English
Subjects:
Adsorption
Analysis
Animals
Biocompatibility
Biomaterials
Biomedical materials
Blood Proteins - analysis
bone regeneration
C-reactive protein
Cell Line, Tumor
Cell Survival - drug effects
Coated Materials, Biocompatible - chemistry
Coated Materials, Biocompatible - toxicity
Dental implants
fibrous capsule
Gels
Gels - chemistry
Gels - toxicity
Haemocompatibility
Humans
Implantation
Implants, Experimental
In vitro methods and tests
In vivo methods and tests
Interactions
Mass spectrometry
Mass spectroscopy
Materials Testing
Mice
Microscopy, Electron, Scanning
Osseointegration
osteoimmunology
Proteins
Proteomics
Rabbits
Silica
Silica gel
Silicon dioxide
Silicon Dioxide - chemistry
Silicon Dioxide - toxicity
Sol-gel processes
Surface Properties
Surgical implants
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Description
Summary:The interactions of implanted biomaterials with the host organism determine the success or failure of an implantation. Normally, their biocompatibility is assessed using in vitro tests. Unfortunately, in vitro and in vivo results are not always concordant; new, effective methods of biomaterial characterisation are urgently needed to predict the in vivo outcome. As the first layer of proteins adsorbed onto the biomaterial surfaces might condition the host response, mass spectrometry analysis was performed to characterise these proteins. Four distinct hybrid sol-gel biomaterials were tested. The in vitro results were similar for all the materials examined here. However, in vivo, the materials behaved differently. Six of the 171 adsorbed proteins were significantly more abundant on the materials with weak biocompatibility; these proteins are associated with the complement pathway. Thus, protein analysis might be a suitable tool to predict the in vivo outcomes of implantations using newly formulated biomaterials.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2017.1356289