Determination of endogenous inflammation-related lipid mediators in ischemic stroke rats using background subtracting calibration curves by liquid chromatography-tandem mass spectrometry

Accurate and reliable quantification of endogenous lipid mediators in complex biological samples is a daunting challenge. In this study, a robust and direct endogenous quantitative method using background subtracting calibration curves by liquid chromatography-tandem mass spectrometry was first deve...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-11, Vol.409 (28), p.6537-6547
Main Authors: Yang, Yang, Zhong, Qisheng, Mo, Canlong, Zhang, Hao, Zhou, Ting, Tan, Wen
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Biochemistry
Biological properties
Biological samples
Brain
Brain Ischemia - pathology
Calibration
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, High Pressure Liquid - methods
Construction standards
Food Science
Health aspects
Infarction, Middle Cerebral Artery - pathology
Inflammation
Inflammation Mediators - analysis
Ischemia
Laboratory Medicine
Lipids
Lipids - analysis
Liquid chromatography
Male
Mass spectrometry
Mass spectroscopy
Methods
Monitoring/Environmental Analysis
Neuroprotection
Physiological aspects
Prostaglandin E2
Prostaglandins
Rats
Rats, Sprague-Dawley
Research Paper
Scientific imaging
Sodium
Spectroscopy
Stroke
Tandem Mass Spectrometry - methods
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Summary:Accurate and reliable quantification of endogenous lipid mediators in complex biological samples is a daunting challenge. In this study, a robust and direct endogenous quantitative method using background subtracting calibration curves by liquid chromatography-tandem mass spectrometry was first developed for the determination of endogenous lipid mediators in ischemic stroke rats. Absolute quantification without surrogate matrix could be achieved by using background subtracting calibration curves, which were corrected and verified from standard curves constructed on original matrix. The recoveries of this method were in the range of 50.3–98.3%, the precision with the relative standard deviation was less than 13.8%, and the accuracy with the relative error was within ± 15.0%. In addition, background subtracting calibration curves were further verified by validation factors ranging from 90.3 to 110.9%. This validated method has been successfully applied to the analysis of seven endogenous inflammation-related lipid mediators in the brain tissues of ischemic stroke rats. The results indicated that prostaglandins as inflammatory factors and some lipid mediators with neuroprotective effects increased apparently ( p  
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-017-0600-7