Site‐specific accumulation and dynamic change of flavonoids in Apocyni Veneti Folium

Site‐specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin‐HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Fol...

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Bibliographic Details
Published in:Microscopy research and technique 2017-12, Vol.80 (12), p.1315-1322
Main Authors: Chen, Cui‐Hua, Xu, Hu, Liu, Xun‐Hong, Zou, Li‐Si, Wang, Mei, Liu, Zi‐Xiu, Fu, Xing‐Sheng, Zhao, Hui, Yan, Ying
Format: Article
Language:English
Subjects:
Accumulation
Apocyni Veneti Folium
Catechin
Cuticles
Derivatives
dynamic change
Flavonoids
Fluorescence
High-performance liquid chromatography
histochemical localization
Kaempferol
Liquid chromatography
Localization
LSCM
Nucleoli
Parenchyma
Protoplasts
Quercetin
Staining
Vanillin
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Summary:Site‐specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin‐HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured using HPLC method. LSCM observation showed that flavonoids are accumulated in cuticle of epidermal cells and vessel walls, especially in protoplasts and nucleolus of the collenchyma cells and the epidermal cells. Catechins are localized in the palisade parenchyma cells and vessel walls, particularly in the laticifers found in the phloem. On the basis of the difference of the maximal emission wavelength between quercetin and kaempferol derivatives which have fluorescence behavior by appropriate treatment, kaempferol and its derivatives are localized exclusively in the cuticle. Results showed that the content of astragalin in Apocyni Veneti Folium from different parts revealed the decreasing trend, while hyperin and isoquercitrin were higher in June and July analyzed by HPLC. In summary, the site‐specific accumulation of flavonoids in Apocyni Veneti Folium can be determined by LSCM and vanillin‐HCl staining. The contents of flavonoids in Apocyni Veneti Folium are correlated with harvest times and growth parts. Site‐specific accumulation of flavonoids was determined by LSCM. Tissue localization of catechins was observed via vanillin‐HCl staining. Dynamic change of flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22943