Isolation and characterization of the trophectoderm from the Arabian camel ( Camelus dromedarius )

Abstract We isolated and characterized trophoblast from in vivo -derived camel embryos and compared with embryonic stem-like cells. Camel embryos were flushed on day 8 post-insemination and used to derive trophectoderm and embryonic stem-like cells under feeder-free culture conditions using a baseme...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2017-09, Vol.57, p.113-122
Main Authors: Saadeldin, Islam M, Swelum, Ayman A, Elsafadi, Mona, Moumen, Abdullah F, Alzahrani, Faisal A, Mahmood, Amer, Alfayez, Musaad, Alowaimer, Abdullah N
Format: Article
Language:English
Subjects:
Animals
Camel
Camelus - embryology
Dromedary
Embryo
Embryo Culture Techniques
Embryo, Mammalian - cytology
Female
Internal Medicine
Obstetrics and Gynecology
Pregnancy
Stem cells
Trophectoderm
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Summary:Abstract We isolated and characterized trophoblast from in vivo -derived camel embryos and compared with embryonic stem-like cells. Camel embryos were flushed on day 8 post-insemination and used to derive trophectoderm and embryonic stem-like cells under feeder-free culture conditions using a basement membrane matrix. Embryos were evaluated for the expression of POU5F1 , MYC , KLF4 , SOX2 , CDX2 , and KRT8 mRNA transcripts by relative quantitative polymerase chain reaction. Camel embryos grew and expanded to ∼4.5 mm and maintained their vesicular shape in vitro for 21 days post-insemination. Trophoblast and embryonic stem-like cell lines grew under feeder-free culture conditions and showed distinct morphological criteria and normal chromosomal counts. Embryonic stem-like cells showed positive staining in the alkaline phosphatase reaction. Trophoblast cells showed a significant increase in CDX2 , KRT8 , KLF4, and SOX2 expression compared with embryonic stem-like cells and whole embryos. Embryonic stem-like cells showed a significant decrease in CDX2 expression and increase in SOX2 and KRT8 expression compared to embryonic expression. POU5F1 and MYC expression showed no difference between embryos and both cell lines. We characterized embryo survival in vitro , particularly the derivation of trophectoderm and embryonic stem-like cells, providing a foundation for further analysis of early embryonic development and placentation in camels.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2017.06.015