Differential expression of albumins and globulins of wheat flours of different technological qualities revealed by nanoUPLC-UDMSE

•Nine common wheat flours were analyzed according different technological qualities.•NanoUPLC-UDMSE was efficient to analyze soluble protein fraction of wheat flour.•Metabolic proteins are differentially expressed in different quality of wheat flour.•Quality-related proteins were up-regulated in the...

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Bibliographic Details
Published in:Food chemistry 2018-01, Vol.239, p.1027-1036
Main Authors: Victorio, Verônica Cristina Mayrinck, Souza, Gustavo H.M.F., Santos, Millena Cristina Barros, Vega, Andrés Rodríguez, Cameron, L.C., Ferreira, Mariana Simões Larraz
Format: Article
Language:English
Subjects:
Domain prediction
Gene ontology
Ion mobility
Proteomic
Triticum aestivum
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Summary:•Nine common wheat flours were analyzed according different technological qualities.•NanoUPLC-UDMSE was efficient to analyze soluble protein fraction of wheat flour.•Metabolic proteins are differentially expressed in different quality of wheat flour.•Quality-related proteins were up-regulated in the two best quality samples.•Principal component analysis clearly distinguished flours of different qualities. Soluble proteins were extracted from common wheat flour obtained from nine cultivars of different qualities and were analyzed by nanoUPLC and Ultra Definition Mass Spectrometry (UDMSE) label-free quantitative approach. Collectively, 5894 proteins were identified and quantified with 8 peptides/protein. A total of 414 proteins were found differentially expressed with 85% proteins not yet described in the literature, according to their biological function. Quality-related proteins, such as puroindolines and chaperones, notably involved in the gluten-protein folding process, were up-regulated in superior (SP) and medium (MD) flours qualities and down-regulated in the low (LW) ones. Domains related to the chaperones activity were also found up-regulated in SP and MD and down-regulated in LW. In this work, nanoUPLC-UDMSE analysis was effective to identify and quantify metabolic proteins, allowing a clear distinction of the wheat flours and providing new perspectives for evaluating the technological quality based on the low abundant proteins of wheat endosperm.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2017.07.049