A 4D view on insulin secretory granule turnover in the β‐cell

Insulin secretory granule (SG) turnover consists of several highly regulated processes allowing for proper β‐cell function and insulin secretion. Besides the spatial distribution of insulin SGs, their age has great impact on the likelihood of their secretion and their behaviour within the β‐cell. Wh...

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Bibliographic Details
Published in:Diabetes, obesity & metabolism obesity & metabolism, 2017-09, Vol.19 (S1), p.107-114
Main Authors: Müller, Andreas, Mziaut, Hassan, Neukam, Martin, Knoch, Klaus‐Peter, Solimena, Michele
Format: Article
Language:English
Subjects:
Animals
Beta cells
Exocytosis
Humans
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Insulin
Insulin - metabolism
insulin degradation
Insulin Secretion
insulin secretory granule ageing
Insulin-Secreting Cells - cytology
Insulin-Secreting Cells - metabolism
Insulin-Secreting Cells - ultrastructure
microscopy
Microscopy, Electron, Transmission - methods
Microscopy, Electron, Transmission - trends
Microscopy, Fluorescence - methods
Microscopy, Fluorescence - trends
Models, Biological
Organelle Biogenesis
Secretory Pathway
Secretory Vesicles - metabolism
Secretory Vesicles - ultrastructure
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Summary:Insulin secretory granule (SG) turnover consists of several highly regulated processes allowing for proper β‐cell function and insulin secretion. Besides the spatial distribution of insulin SGs, their age has great impact on the likelihood of their secretion and their behaviour within the β‐cell. While quantitative measurements performed decades ago demonstrated the preferential secretion of young insulin, new experimental approaches aim to investigate insulin ageing at the granular level. Live‐cell imaging, automated image analysis and correlative light and electron microscopy have fostered knowledge of age‐defined insulin SG dynamics, their interaction with the cytoskeleton and ultrastructural features. Here, we review our recent work in regards to the connection between insulin SG age, SG dynamics, intracellular location and interaction with other proteins.
ISSN:1462-8902
1463-1326
DOI:10.1111/dom.13015