A novel MFS transporter encoding gene in Fusarium verticillioides probably involved in iron-siderophore transport

The major facilitator superfamily (MFS) is a ubiquitous group of proteins involved in the transport of a wide range of compounds, including toxins produced by fungal species. In this paper, a novel MFS encoding gene ( Fusarium iron related gene or FIR1), which had shown an up-regulation in fumonisin...

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Bibliographic Details
Published in:Mycological research 2006-09, Vol.110 (9), p.1102-1110
Main Authors: LÓPEZ-ERRASQUÍN, Elena, GONZÁLEZ-JAÉN, M. Teresa, CALLEJAS, Carmen, VáZQUEZ, Covadonga
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
F. Verticillioides
Fumonisins
Fumonisins - metabolism
fungal proteins
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fusarium
Fusarium - genetics
Fusarium - growth & development
Fusarium - metabolism
Fusarium verticillioides
Gibberella fujikuroi
Iron - metabolism
major facilitator superfamily
Membrane Transport Proteins - chemistry
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
MFS transporter
molecular sequence data
nucleotide sequences
Phylogeny
plant pathogenic fungi
Real-time RT-PCR
Sequence Analysis, DNA
Siderophore transporter
siderophores
Siderophores - metabolism
transmembrane proteins
transport proteins
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Summary:The major facilitator superfamily (MFS) is a ubiquitous group of proteins involved in the transport of a wide range of compounds, including toxins produced by fungal species. In this paper, a novel MFS encoding gene ( Fusarium iron related gene or FIR1), which had shown an up-regulation in fumonisin-inducing conditions, has been identified and characterized. The deduced protein sequence, which predicted 14 transmembrane domains typical of MFS transporters and its phylogenetic relationships with representative members of MFS transporters suggested a possible function of FIR1 as a siderophore transporter. A real-time RT-PCR protocol has been developed to analyse the expression pattern of the FIR1 gene in relation to siderophore production. The results indicated that the synthesis of extracellular siderophores by F. verticillioides observed in absence of extracellular iron was repressed in iron-supplemented cultures and showed a good correspondence with FIR1 gene expression. However, the pattern of FIR1 gene expression observed suggested that this gene did not seem to be functionally related to fumonisin production.
ISSN:0953-7562
1469-8102
DOI:10.1016/j.mycres.2006.06.009