Adsorption effects of the doping relevant peptides Insulin Lispro, Synachten, TB-500 and GHRP 5

The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant mo...

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Bibliographic Details
Published in:Analytical biochemistry 2017-11, Vol.537, p.69-71
Main Authors: Judák, Péter, Van Eenoo, Peter, Deventer, Koen
Format: Article
Language:English
Subjects:
Adsorption
Animals
Chromatography, High Pressure Liquid
Cosyntropin - blood
Cosyntropin - chemistry
Doping control
Doping in Sports
Glass - chemistry
Humans
Insulin Lispro - blood
Insulin Lispro - chemistry
Low-bind
Oligopeptides - blood
Oligopeptides - chemistry
Peptide
Polypropylenes - chemistry
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Summary:The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant model peptides (GHRP 5, TB-500, Insulin Lispro, Synachten) were chosen and their recovery from various surfaces were evaluated. Our experiments showed that choosing expensive consumables with low-bind characteristics is not beneficial in all cases. A careful selection of the consumables based on the evaluation of the physico/chemical features of the peptide is recommended. [Display omitted]
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2017.09.003