Novel amine-functionalized iron trimesates with enhanced peroxidase-like activity and their applications for the fluorescent assay of choline and acetylcholine

• Published in: Biosensors & bioelectronics 2018-02, Vol.100, p.161-168
• Main Authors: Valekar, Anil H., Batule, Bhagwan S., Kim, Moon Il, Cho, Kyung-Ho, Hong, Do-Young, Lee, U-Hwang, Chang, Jong-San, Park, Hyun Gyu, Hwang, Young Kyu
• Format: Article
• Language: English
• Subjects: Acetylcholine - analysis; Acetylcholine - blood; Acetylcholine detection; Amination; Amine grafting; Animals; Biosensing Techniques - methods; Catalysis; Choline - analysis; Choline - blood; Choline detection; Humans; Iron Compounds - chemistry; Metal–organic framework; Milk - chemistry; Models, Molecular; Organometallic Compounds - chemistry; Peroxidase - chemistry; Peroxidase-like activity; Putrescine - analogs & derivatives; Putrescine - chemistry; Spectrometry, Fluorescence - methods
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.08.056

We herein describe novel amine-grafted metal–organic frameworks (MOFs) as a promising alternative to natural peroxidase enzyme and their applications for a fluorescent assay of choline (Cho) and acetylcholine (ACh). Among diverse amine-functionalized MOFs, N,N,N′,N′-tetramethyl-1,4-butanediamine (TMBDA)-functionalized MIL-100(Fe) (TMBDA-MIL-100(Fe)) exhibited the highest peroxidase activity by developing intense fluorescence from Amplex UltraRed (AUR) in the presence of H2O2, which was presumably due to the synergetic effect of the enhanced negative potential and precisely controlled molecular size of the grafted diamine. Based on the excellent peroxidase-like activity of TMBDA-MIL-100(Fe), choline and ACh were reliably determined down to 0.027 and 0.036µM, respectively. Furthermore, practical applicability of this strategy was successfully demonstrated by detecting choline and ACh in spiked samples of milk and serum, respectively. This work highlights the advantages of amine-grafted MOFs for the preparation of biomimetic catalysts, extending their scope to biosensor applications. •Various aliphatic diamines were grafted on coordinatively unsaturated metals sites of MIL-100(Fe) and subjected for peroxidase-like activity.•Based on the excellent peroxidase-like activity of AFM, a simple and sensitive fluorescent detection of choline and acetylcholine was successfully performed.•Effectively determined choline and acetylcholine levels in real samples of milk and serum, respectively.