Inhibition of hepatitis B virus by D-fraction from Grifola frondosa: Synergistic effect of combination with interferon-α in HepG2 2.2.15

In this study, D-fraction extracted from Grifola frondosa (GF-D) and its combination with human interferon alpha-2b (IFN) were investigated for the inhibitory effect on hepatitis B virus (HBV) in HepG2 2.2.15 cells (2.2.15 cells). HBV DNA and viral antigens were analyzed by a quantitative real-time...

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Bibliographic Details
Published in:Antiviral research 2006-11, Vol.72 (2), p.162-165
Main Authors: Gu, Chang-Qing, Li, Jun-Wen, Chao, Fu-Huan
Format: Article
Language:English
Subjects:
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral
Antiviral agents
Antiviral Agents - isolation & purification
Antiviral Agents - pharmacology
Biological and medical sciences
Cell Line, Tumor
Combination
DNA, Viral - analysis
Drug Synergism
Grifola - chemistry
Grifola frondosa
Hepatitis B Antigens - analysis
Hepatitis B virus
Hepatitis B virus - drug effects
Hepatitis B virus - physiology
Human viral diseases
Humans
Infectious diseases
Interferon
Interferon-alpha - pharmacology
Medical sciences
Pharmacology. Drug treatments
Polymerase Chain Reaction
Recombinant Proteins
Viral diseases
Viral hepatitis
Virus Replication - drug effects
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Summary:In this study, D-fraction extracted from Grifola frondosa (GF-D) and its combination with human interferon alpha-2b (IFN) were investigated for the inhibitory effect on hepatitis B virus (HBV) in HepG2 2.2.15 cells (2.2.15 cells). HBV DNA and viral antigens were analyzed by a quantitative real-time polymerase chain reaction and end-point titration in radioimmunoassays, respectively. The results showed that GF-D or IFN alone could inhibit HBV DNA in 2.2.15 cells with the 50% inhibitory concentration (IC 50) of 0.59 mg/ml and 1399 IU/ml, respectively. We further investigated the combination of GF-D and IFN for anti-HBV activity and found that they synergistically inhibited HBV replication in 2.2.15 cells. In combination with 0.45 mg/ml GF-D, the apparent IC 50 value for IFN was 154 IU/ml. This 9-fold increase in antiviral activity of IFN suggested that GF-D could synergize with IFN. These results indicate that GF-D, in combination with IFN, might provide a potentially effective therapy against chronic HBV infections.
ISSN:0166-3542
1872-9096
DOI:10.1016/j.antiviral.2006.05.011