Dynamics and function of ERM proteins during cytokinesis in human cells

The molecular mechanism that governs cytoskeleton–membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furro...

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Bibliographic Details
Published in:FEBS letters 2017-10, Vol.591 (20), p.3296-3309
Main Authors: Hiruma, Shota, Kamasaki, Tomoko, Otomo, Kohei, Nemoto, Tomomi, Uehara, Ryota
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Actin Cytoskeleton - ultrastructure
Cell Line, Transformed
Cell Membrane - metabolism
Cell Membrane - ultrastructure
cytokinesis
Cytokinesis - genetics
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Ezrin/Radixin/Moesin
Gene Expression Regulation
Genes, Reporter
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
Luciferases - genetics
Luciferases - metabolism
Membrane Proteins - genetics
Membrane Proteins - metabolism
Microfilament Proteins - genetics
Microfilament Proteins - metabolism
Molecular Dynamics Simulation
rhoA GTP-Binding Protein - genetics
rhoA GTP-Binding Protein - metabolism
Signal Transduction
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Summary:The molecular mechanism that governs cytoskeleton–membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane‐associated domain in a cholesterol‐dependent but largely Rho‐independent manner. While ERMs are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar cortex. Notably, when anillin and supervillin are codepleted, ERMs increasingly accumulate at the cleavage furrow and substantially contribute to the furrow ingression. These results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis‐relevant proteins with the division site.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12844