Different impacts of manure and chemical fertilizers on bacterial community structure and antibiotic resistance genes in arable soils

Both manure and chemical fertilizers are widely used in modern agriculture. However, the impacts of different fertilizers on bacterial community structure and antibiotic resistance genes (ARGs) in arable soils still remain unclear. In this study, high-throughput sequencing and quantitative PCR were...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2017-12, Vol.188, p.455-464
Main Authors: Liu, Peng, Jia, Shuyu, He, Xiwei, Zhang, Xuxiang, Ye, Lin
Format: Article
Language:English
Subjects:
Agriculture - methods
Antibiotic resistance genes
Arable soil
Bacterial community
China
Drug Resistance, Microbial - genetics
Fertilizer application
Fertilizers
Genes, Bacterial
High-Throughput Screening Assays
Manure
Metagenomics
Microbiota - drug effects
Microbiota - genetics
Soil - chemistry
Soil Microbiology - standards
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Summary:Both manure and chemical fertilizers are widely used in modern agriculture. However, the impacts of different fertilizers on bacterial community structure and antibiotic resistance genes (ARGs) in arable soils still remain unclear. In this study, high-throughput sequencing and quantitative PCR were employed to investigate the bacterial community structure, ARGs and mobile genetic elements (MGEs) influenced by the application of different fertilizers, including chemical fertilizers, piggery manure and straw ash. The results showed that the application of fertilizers could significantly change the soil bacterial community and the abundance of Gaiella under phylum Actinobacteria was significantly reduced from 12.9% in unfertilized soil to 4.1%–7.4% in fertilized soil (P 
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2017.08.162