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Fast technique for the identification of Gilbertella persicaria via optical microscopy
Gilbertella persicaria is an important phytopathogen that is confused with Mucor spp. and Rhizopus spp. The main distinguishing characteristic of G. persicaria is the presence of appendages in sporangiospores, and their observation by conventional staining techniques generally fails. A technique is...
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| Published in: | Journal of microbiological methods 2017-11, Vol.142, p.36-38 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c359t-d367d842b635a4829765c8c127d9b06d0c166363f66439b2b6d671cabcff6db13 |
| container_end_page | 38 |
| container_issue | |
| container_start_page | 36 |
| container_title | Journal of microbiological methods |
| container_volume | 142 |
| creator | Cruz-Lachica, Isabel Marquez-Zequera, Isidro Allende-Molar, Raul Sañudo-Barajas, Josefa Adriana Leon-Felix, Josefina Garcia-Estrada, Raymundo Saul |
| description | Gilbertella persicaria is an important phytopathogen that is confused with Mucor spp. and Rhizopus spp. The main distinguishing characteristic of G. persicaria is the presence of appendages in sporangiospores, and their observation by conventional staining techniques generally fails. A technique is described using light microscopy for fast and reliable diagnosis.
•Gilbertella persicaria is a frequent causal agent of pre- and post-harvest losses.•Identification of Gilbertella persicaria is highly susceptible to errors.•A technique to quickly and reliably identify Gilbertella persicaria was developed.•This technique can be used for both brightfield and darkfield. |
| doi_str_mv | 10.1016/j.mimet.2017.09.004 |
| format | article |
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•Gilbertella persicaria is a frequent causal agent of pre- and post-harvest losses.•Identification of Gilbertella persicaria is highly susceptible to errors.•A technique to quickly and reliably identify Gilbertella persicaria was developed.•This technique can be used for both brightfield and darkfield.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2017.09.004</identifier><identifier>PMID: 28899635</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Choanephoraceae ; Darkfield ; DIC microscopy ; Microscopy - methods ; Mucor irregularis ; Mucorales - classification ; Mucormycosis - diagnosis ; Mucormycosis - microbiology ; Plant Diseases - microbiology ; Rhizopus oryzae ; Spores, Fungal</subject><ispartof>Journal of microbiological methods, 2017-11, Vol.142, p.36-38</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-d367d842b635a4829765c8c127d9b06d0c166363f66439b2b6d671cabcff6db13</citedby><cites>FETCH-LOGICAL-c359t-d367d842b635a4829765c8c127d9b06d0c166363f66439b2b6d671cabcff6db13</cites><orcidid>0000-0001-6129-8128</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28899635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cruz-Lachica, Isabel</creatorcontrib><creatorcontrib>Marquez-Zequera, Isidro</creatorcontrib><creatorcontrib>Allende-Molar, Raul</creatorcontrib><creatorcontrib>Sañudo-Barajas, Josefa Adriana</creatorcontrib><creatorcontrib>Leon-Felix, Josefina</creatorcontrib><creatorcontrib>Garcia-Estrada, Raymundo Saul</creatorcontrib><title>Fast technique for the identification of Gilbertella persicaria via optical microscopy</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>Gilbertella persicaria is an important phytopathogen that is confused with Mucor spp. and Rhizopus spp. The main distinguishing characteristic of G. persicaria is the presence of appendages in sporangiospores, and their observation by conventional staining techniques generally fails. A technique is described using light microscopy for fast and reliable diagnosis.
•Gilbertella persicaria is a frequent causal agent of pre- and post-harvest losses.•Identification of Gilbertella persicaria is highly susceptible to errors.•A technique to quickly and reliably identify Gilbertella persicaria was developed.•This technique can be used for both brightfield and darkfield.</description><subject>Choanephoraceae</subject><subject>Darkfield</subject><subject>DIC microscopy</subject><subject>Microscopy - methods</subject><subject>Mucor irregularis</subject><subject>Mucorales - classification</subject><subject>Mucormycosis - diagnosis</subject><subject>Mucormycosis - microbiology</subject><subject>Plant Diseases - microbiology</subject><subject>Rhizopus oryzae</subject><subject>Spores, Fungal</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMo7rr6CwTJ0Utr0nTzcfAg4hcseFGvoU2mbJa2qUlW8N-bddWjhzCEPDPz5EXonJKSEsqvNuXgBkhlRagoiSoJqQ_QnEpRFZIt1SGaZ0oUgtBqhk5i3BBCl6yWx2hWSakUZ8s5ertvYsIJzHp071vAnQ84rQE7C2NynTNNcn7EvsMPrm8hJOj7Bk8QYn4KrsEf-fgp5VuPB2eCj8ZPn6foqGv6CGc_dYFe7-9ebh-L1fPD0-3NqjDZMBWWcWFlXbXZpallpQRfGmloJaxqCbfEUM4ZZx3nNVNt5iwX1DSt6TpuW8oW6HI_dwo-68ekBxfNznEEv42aKiY5obVSGWV7dOcYA3R6Cm5owqemRO8C1Rv9HajeBaqJ0jnQ3HXxs2DbDmD_en4TzMD1HoD8zQ8HQUfjYDRgXQCTtPXu3wVfmsaIsw</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Cruz-Lachica, Isabel</creator><creator>Marquez-Zequera, Isidro</creator><creator>Allende-Molar, Raul</creator><creator>Sañudo-Barajas, Josefa Adriana</creator><creator>Leon-Felix, Josefina</creator><creator>Garcia-Estrada, Raymundo Saul</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6129-8128</orcidid></search><sort><creationdate>201711</creationdate><title>Fast technique for the identification of Gilbertella persicaria via optical microscopy</title><author>Cruz-Lachica, Isabel ; Marquez-Zequera, Isidro ; Allende-Molar, Raul ; Sañudo-Barajas, Josefa Adriana ; Leon-Felix, Josefina ; Garcia-Estrada, Raymundo Saul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-d367d842b635a4829765c8c127d9b06d0c166363f66439b2b6d671cabcff6db13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Choanephoraceae</topic><topic>Darkfield</topic><topic>DIC microscopy</topic><topic>Microscopy - methods</topic><topic>Mucor irregularis</topic><topic>Mucorales - classification</topic><topic>Mucormycosis - diagnosis</topic><topic>Mucormycosis - microbiology</topic><topic>Plant Diseases - microbiology</topic><topic>Rhizopus oryzae</topic><topic>Spores, Fungal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cruz-Lachica, Isabel</creatorcontrib><creatorcontrib>Marquez-Zequera, Isidro</creatorcontrib><creatorcontrib>Allende-Molar, Raul</creatorcontrib><creatorcontrib>Sañudo-Barajas, Josefa Adriana</creatorcontrib><creatorcontrib>Leon-Felix, Josefina</creatorcontrib><creatorcontrib>Garcia-Estrada, Raymundo Saul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cruz-Lachica, Isabel</au><au>Marquez-Zequera, Isidro</au><au>Allende-Molar, Raul</au><au>Sañudo-Barajas, Josefa Adriana</au><au>Leon-Felix, Josefina</au><au>Garcia-Estrada, Raymundo Saul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fast technique for the identification of Gilbertella persicaria via optical microscopy</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2017-11</date><risdate>2017</risdate><volume>142</volume><spage>36</spage><epage>38</epage><pages>36-38</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>Gilbertella persicaria is an important phytopathogen that is confused with Mucor spp. and Rhizopus spp. The main distinguishing characteristic of G. persicaria is the presence of appendages in sporangiospores, and their observation by conventional staining techniques generally fails. A technique is described using light microscopy for fast and reliable diagnosis.
•Gilbertella persicaria is a frequent causal agent of pre- and post-harvest losses.•Identification of Gilbertella persicaria is highly susceptible to errors.•A technique to quickly and reliably identify Gilbertella persicaria was developed.•This technique can be used for both brightfield and darkfield.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28899635</pmid><doi>10.1016/j.mimet.2017.09.004</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0001-6129-8128</orcidid></addata></record> |
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| ispartof | Journal of microbiological methods, 2017-11, Vol.142, p.36-38 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Choanephoraceae Darkfield DIC microscopy Microscopy - methods Mucor irregularis Mucorales - classification Mucormycosis - diagnosis Mucormycosis - microbiology Plant Diseases - microbiology Rhizopus oryzae Spores, Fungal |
| title | Fast technique for the identification of Gilbertella persicaria via optical microscopy |
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