H+ translocation by weak acid uncouplers is independent of H+ electrochemical gradient

According to the common view, weak acid uncouplers increase proton conductance of biological (and phospholipid bilayer) membranes, thus effecting H + fluxes driven by their electrochemical gradients. Under certain conditions, however, uncouplers can induce unexpected effects opposite to the dissipat...

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Bibliographic Details
Published in:Journal of bioenergetics and biomembranes 2017-10, Vol.49 (5), p.391-397
Main Authors: Plášek, Jaromír, Babuka, David, Hoefer, Milan
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biochemistry
Biological Transport - drug effects
Bioorganic Chemistry
Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology
Chemistry
Chemistry and Materials Science
Histology
Hydrogen-Ion Concentration - drug effects
Membrane Potentials - drug effects
Morphology
Organic Chemistry
Protons
Saccharomyces cerevisiae - metabolism
Uncoupling Agents - pharmacology
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Summary:According to the common view, weak acid uncouplers increase proton conductance of biological (and phospholipid bilayer) membranes, thus effecting H + fluxes driven by their electrochemical gradients. Under certain conditions, however, uncouplers can induce unexpected effects opposite to the dissipation of H + gradients. Results are presented here demonstrating CCCP-induced proton influx into Saccharomyces cerevisiae cytosol driven by the electrochemical potentials of CCCP and its CCCP − anions, independent of electrochemical H + -gradient. Another view of week acid uncouplers’ action is proposed that is logically consistent with these observations.
ISSN:0145-479X
1573-6881
DOI:10.1007/s10863-017-9724-x