Effectiveness of alternative organic solvents in field preservation of whole barnacles for PCR analyses

There are few reports of non-cryogenic preservation methods for marine invertebrates, so potable alcohol and acetone-based nail varnish remover (NVR) are for the first time evaluated against absolute ethanol as short-term preservatives of whole barnacles. Performance of ethanol and NVR-preserved mat...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the Marine Biological Association of the United Kingdom 2004-08, Vol.84 (4), p.747-749
Main Authors: Lee, P.L.M., Beynon, C.M.
Format: Article
Language:English
Subjects:
Acetone
Alcohol
Animal, plant and microbial ecology
Biological and medical sciences
Cryopreservation
Ethanol
Fieldwork
Fundamental and applied biological sciences. Psychology
General aspects. Techniques
Invertebrates
Laboratories
Marine
Marine invertebrates
Methods and techniques (sampling, tagging, trapping, modelling...)
Mitochondrial DNA
Molecular weight
Organic solvents
Preservatives
Research Article
Solvents
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There are few reports of non-cryogenic preservation methods for marine invertebrates, so potable alcohol and acetone-based nail varnish remover (NVR) are for the first time evaluated against absolute ethanol as short-term preservatives of whole barnacles. Performance of ethanol and NVR-preserved material was comparable, but potable alcohol was significantly worse. These results are of practical importance for fieldwork in remote areas where laboratory chemicals are unattainable but potable alcohol or NVR are locally available. Of these, acetone-based NVR would be the solvent of preference.
ISSN:0025-3154
1469-7769
DOI:10.1017/S0025315404009853h