RESPIRATORY BURST OF BIOMPHALARIA GLABRATA HEMOCYTES: SCHISTOSOMA MANSONI–RESISTANT SNAILS PRODUCE MORE EXTRACELLULAR H2O2 THAN SUSCEPTIBLE SNAILS

• Published in: The Journal of parasitology 2005-04, Vol.91 (2), p.275-279
• Main Authors: Bender, Randall C, Broderick, Erica J, Goodall, Cheri P, Bayne, Christopher J
• Format: Article
• Language: English
• Subjects: 12-O-Tetradecanoylphorbol-13-acetate; Acetic acid; Animals; Biological and medical sciences; Biomphalaria - immunology; Biomphalaria - metabolism; Biomphalaria - parasitology; Biomphalaria glabrata; Disease Susceptibility; Experiments; Fundamental and applied biological sciences. Psychology; Gastropoda; General aspects; General aspects and techniques. Study of several systematic groups. Models; Hemocytes; Hemocytes - immunology; Hemocytes - metabolism; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Immunity, Innate; INVERTEBRATE-PARASITE RELATIONSHIPS; Invertebrates; Kinases; Marine; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; Mollusks; Parasites; Plasma; Protein kinase C; Protein Kinase C - metabolism; Reactive oxygen species; Respiratory burst; Respiratory Burst - immunology; Respiratory Burst - physiology; Schistosoma mansoni; Schistosoma mansoni - immunology; Signal transduction; Snails; Tetradecanoylphorbol Acetate - pharmacology; Vectors. Intermediate hosts
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-415R

The production of reactive oxygen species by hemocytes from the gastropod Biomphalaria glabrata has been linked to their ability to kill the trematode parasite Schistosoma mansoni. For 2 laboratory strains of the snail, 1 resistant (13-16-R1) and 1 susceptible (MO) to the PR1 strain of S. mansoni, we compared hemocyte production of extracellular hydrogen peroxide when stimulated with the protein kinase C agonist phorbol myristate acetate (PMA). The time course of the PMA-induced response is similar in both strains with respect to onset, peak production, and termination of the respiratory burst. However, the magnitude of the response differs between strains, in that hemocytes from resistant snails generate significantly more hydrogen peroxide. These findings suggest that the capacity to produce hydrogen peroxide could be critical in determining susceptibility or resistance to S. mansoni.