PRIMER NOTE: Polymerase chain reaction primers for polymorphic microsatellite loci from the Korean sandfish, Arctoscopus japonicus

We developed 12 polymorphic microsatellite markers from Arctoscopus japonicus by screening an enriched genomic library using polymerase chain reaction (PCR) techniques. The average of alleles size was 16.2, and the average observed and expected heterozygosities were 0.59 and 0.78, respectively. The...

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Bibliographic Details
Published in:Molecular ecology notes 2006-09, Vol.6 (3), p.674-676
Main Authors: Park, Jung Youn, Lee, Sang-Jun, Lee, Hae Won, Lee, Yong Gug, Jung, So Jung, Kang, Yong Joo
Format: Article
Language:English
Subjects:
Arctoscopus japonicus
Marine
Online Access:Get full text
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Summary:We developed 12 polymorphic microsatellite markers from Arctoscopus japonicus by screening an enriched genomic library using polymerase chain reaction (PCR) techniques. The average of alleles size was 16.2, and the average observed and expected heterozygosities were 0.59 and 0.78, respectively. The observed genotypic frequencies in five loci were significantly deviated from Hardy-Weinberg expectations. The high variability revealed in this study suggested that these microsatellite loci should provide useful markers for population genetics of A. japonicus.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2006.01298.x