Interferon activates promoter of Nmi gene via interferon regulator factor-1

N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated. We identified an...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2018-04, Vol.441 (1-2), p.165-171
Main Authors: Xu, Xiao, Chai, Keli, Chen, Yuhang, Lin, Yongquan, Zhang, Suzhen, Li, Xin, Qiao, Wentao, Tan, Juan
Format: Article
Language:English
Subjects:
Analysis
Biochemistry
Biological response modifiers
Biomedical and Life Sciences
Cardiology
Cellular signal transduction
Gene regulation
Genetic aspects
Interferon
Interferon regulatory factor
Interferon regulatory factor 1
Life Sciences
Luciferase
Medical Biochemistry
Myc protein
Nmi protein
Oncology
Signal transduction
Signaling
Transcription
Transcription (Genetics)
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Summary:N-Myc interactor (Nmi) is reported to participate in many activities, such as signaling transduction, transcription regulation, and antiviral responses. As Nmi may play important roles in interferon (IFN)-induced responses, we investigated the mechanism how Nmi protein is regulated. We identified and cloned the promoter of Nmi gene. Sequence analysis and luciferase assays shown that an IFN-stimulated response element (ISRE) and a GC box in the promoter were essential for the basal transcription activity of Nmi gene. We also found that interferon regulatory factor 1 (IRF-1) could activate transcription of Nmi by binding to the ISRE in the promoter. Knockdown of IRF-1 decreases IFN-induced Nmi transcription. These results revealed that IRF-1 is involved in the IFN-inducible expression of Nmi.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-017-3182-y