Mast cell exosomes can suppress allergic reactions by binding to IgE

• Published in: Journal of allergy and clinical immunology 2018-02, Vol.141 (2), p.788-791
• Main Authors: Xie, Guogang, Yang, Haiwei, Peng, Xia, Lin, Lihui, Wang, Juan, Lin, Kun, Cui, Zelin, Li, Jia, Xiao, Hui, Liang, Yuting, Li, Li
• Format: Article
• Language: English
• Subjects: Allergies; Antigens; Asthma; Cytokines; Exosomes; Hypersensitivity; Immunoglobulin E; Kinases; Proteins; Rodents; Smooth muscle
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2017.07.040

Because exosomes derived from Fc[epsilon]RIα−/− BMMCs failed to show the ability of binding to IgE, it was believed that BMMC exosomes bound to IgE via the receptor Fc[epsilon]RI. Furthermore, Western blotting demonstrated that phospholipaseCγ1 and protein kinase C decreased phosphorylation when BMMC exosomes were added, compared with IgE alone or Fc[epsilon]RIα−/− BMMCs exosomes. [...]BMMC exosomes might inhibit the activation of MCs through the phospholipase Cγ1-protein kinase C pathway, which was regarded as the “principle” signaling cascade in activated MCs.8 We then developed a mouse model of allergic asthma induced by exposure to ovalbumin (OVA) as previously described.9 The mice were divided into 5 groups (n = 12 per group) as control group: mice were sensitized and challenged with PBS; OVA group: mice were sensitized and challenged with OVA; OVA + exo for 1 month, 2 months, and 3 months group: mice were sensitized and challenged with OVA and followed up with treatment with BMMC exosomes for 1, 2, and 3 months, respectively. [...]BMMC exosomes possess anti-IgE effect, which decreases IgE levels and inhibits MC activation.