Biological functions of antioxidants in plant transformation

Browning and necrosis of transformed cells/tissues, and difficulty to regenerate transgenic plants from the transformed cells/tissues (recalcitrance) are common in Agrobacterium-mediated transformation process in many plant species. In addition, most crop transformation methods that use NPTII select...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2008-06, Vol.44 (3), p.149-161
Main Author: Dan, Yinghui
Format: Article
Language:English
Subjects:
Agrobacterium
Antioxidants
Apoptosis
Biomedical and Life Sciences
Cell Biology
Cells
Coculture techniques
Developmental Biology
Flowers & plants
Free radicals
Genetic engineering
Genetic Transformation
Hydrogen peroxide
Hydroxyl radicals
INVITED REVIEW
Life Sciences
Molecular weight
Necrosis
Oxidative burst
Plant Breeding/Biotechnology
Plant cells
Plant Genetics and Genomics
Plant Sciences
Plant species
Plant tissues
Plants
Proteins
Reactive oxygen species
shoots
superoxide anion
Tissues
Transgenic plants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Browning and necrosis of transformed cells/tissues, and difficulty to regenerate transgenic plants from the transformed cells/tissues (recalcitrance) are common in Agrobacterium-mediated transformation process in many plant species. In addition, most crop transformation methods that use NPTII selection produce a significant number of nontransgenic shoots, called “shoot escapes” even under stringent selection conditions. These common problems of plant transformation, (browning and necrosis of transformed cells/tissues, recalcitrance, and the occurrence of shoot escapes) severely reduces transformation efficiency. Recent research indicates that reactive oxygen species (ROS) such as superoxide radical (O2−), the hydrogen peroxide (H2O2), the hydroxyl radical (OH'), and the peroxyl radical (RO2) may be playing an important role in tissue browning and necrosis during transformation. This review examines the role of ROS in in vitro recalcitrance and genetic transformation and the opportunities to improve transformation efficiency using antioxidants.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-008-9110-9