Interleukin-10 Gene Transfer in Rat Limbal Transplantation

Purpose: To evaluate the gene transfer of the interleukin (IL)-10 cytokine as a treatment modality for prolonging limbal allograft survival in a rat model. Materials and methods: Adenoviral (AV) and lentiviral (LV) vectors were produced for ex vivo gene transfer into limbal graft tissue prior to ort...

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Bibliographic Details
Published in:Current eye research 2017-11, Vol.42 (11), p.1426-1434
Main Authors: Kaufmann, Claude, Mortimer, Lauren A, Brereton, Helen M, Irani, Yazad D, Parker, Douglas GA, Anson, Donald S, Bachmann, Lucas M, Williams, Keryn A
Format: Article
Language:English
Subjects:
Adenoviral vector
Animals
Cells, Cultured
Corneal Diseases - pathology
Corneal Diseases - surgery
Corneal Transplantation - methods
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Follow-Up Studies
Gene Expression Regulation
gene therapy
Gene Transfer Techniques
Graft Rejection - genetics
Graft Rejection - pathology
Graft Rejection - prevention & control
Graft Survival - genetics
interleukin-10
Interleukin-10 - biosynthesis
Interleukin-10 - genetics
lentiviral vector
limbal transplantation
Limbus Corneae - cytology
Limbus Corneae - surgery
Rats
Rats, Inbred F344
Rats, Inbred WF
Reverse Transcriptase Polymerase Chain Reaction
RNA - genetics
Transplantation, Homologous
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Summary:Purpose: To evaluate the gene transfer of the interleukin (IL)-10 cytokine as a treatment modality for prolonging limbal allograft survival in a rat model. Materials and methods: Adenoviral (AV) and lentiviral (LV) vectors were produced for ex vivo gene transfer into limbal graft tissue prior to orthotopic transplantation. Experimental groups comprised unmodified isografts, unmodified allografts, allografts transfected with a reporter gene, and allografts transfected with IL-10. The functional effects of the transgenes were determined by clinical assessment and by following donor cell survival in the recipient animal. Group comparisons were made using survival analysis and tested with the log-rank test. Differences in mean rejection times between groups were tested using the Wilcoxon rank-sum test. Results: Isografts survived during the entire observation period of 56 days. Allografts underwent clinical rejection at a mean of 6.7 days (standard deviation 2.0) postoperatively, irrespective of the presence of transgenes (p 
ISSN:0271-3683
1460-2202
DOI:10.1080/02713683.2017.1344714