Regenerating Heart Using a Novel Compound and Human Wharton Jelly Mesenchymal Stem Cells

Myocardial infarction is a major problem in health system and most conventional therapy is not led to restoration of the health. Stem cell therapy is a method to regenerate the heart but today appropriate cell source and scaffold selection as extracellular matrix to achieve the best effect is disput...

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Bibliographic Details
Published in:Archives of medical research 2017-04, Vol.48 (3), p.228-237
Main Authors: Rabbani, Shahram, Soleimani, Masoud, Imani, Mohammad, Sahebjam, Mohammad, Ghiaseddin, Ali, Nassiri, Seyed Mahdi, Majd Ardakani, Jalil, Tajik Rostami, Maryam, Jalali, Arash, Mousanassab, Bahmanshir, Kheradmandi, Mahsa, Ahmadi Tafti, Seyed Hossein
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Cell Differentiation
Cell therapy
Chitosan
Humans
Hyaluronic Acid
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells - cytology
Myocardial infarction
Myocardial Infarction - pathology
Myocardial Infarction - physiopathology
Myocardial Infarction - therapy
Myocardium - pathology
Neovascularization, Physiologic
Polyethylene Glycols
Rabbit
Rabbits
Regeneration
Scaffold
Tissue Scaffolds
Wharton Jelly
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Summary:Myocardial infarction is a major problem in health system and most conventional therapy is not led to restoration of the health. Stem cell therapy is a method to regenerate the heart but today appropriate cell source and scaffold selection as extracellular matrix to achieve the best effect is disputing. In this study a combination of human Wharton jelly mesenchymal stem cells (HWJMSCs) with a novel compound consisting polyethylene glycol (PEG), hyaluronic acid and chitosan is presented to heart regeneration. After proliferation and expansion of HWJMSCs, these cells were mixed with scaffold and injected into the infarcted rabbit myocardium. After two months cardiac function and infarcted area were evaluated. Immunohistochemistry performed for vessel count and demonstrating of differentiation ability into cardiomyocytes. To confirm this ability PCR was done. Scanning electron microscope was used to evaluate angiogenesis. Improving cardiac function was higher in cell/scaffold group than the others and it was confirmed by SPECT results which showed least defect size in the myocardium. There were a lot of neoangiogenesis in the target group and also cardiomyogenesis observed in cell/scaffold group. PCR results confirmed the presence of differentiated cardiomyocytes and SEM showed well developed vessel in this group. Comparing macroscopic and microscopic results between all groups revealed that HWJMSC in combination with this scaffold led to brilliant results regarding cardiac function, angiogenesis and cardiogenesis. It is recommended using these cells and materials for cardiac tissue engineering and regeneration therapy.
ISSN:0188-4409
1873-5487
DOI:10.1016/j.arcmed.2017.03.019