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Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan

Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known abo...

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Published in:Infection, genetics and evolution genetics and evolution, 2017-11, Vol.55, p.209-217
Main Authors: Kuroda, Moegi, Masuda, Tsuneyuki, Ito, Mika, Naoi, Yuki, Doan, Yen Hai, Haga, Kei, Tsuchiaka, Shinobu, Kishimoto, Mai, Sano, Kaori, Omatsu, Tsutomu, Katayama, Yukie, Oba, Mami, Aoki, Hiroshi, Ichimaru, Toru, Sunaga, Fujiko, Mukono, Itsuro, Yamasato, Hiroshi, Shirai, Junsuke, Katayama, Kazuhiko, Mizutani, Tetsuya, Oka, Tomoichiro, Nagai, Makoto
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container_title Infection, genetics and evolution
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creator Kuroda, Moegi
Masuda, Tsuneyuki
Ito, Mika
Naoi, Yuki
Doan, Yen Hai
Haga, Kei
Tsuchiaka, Shinobu
Kishimoto, Mai
Sano, Kaori
Omatsu, Tsutomu
Katayama, Yukie
Oba, Mami
Aoki, Hiroshi
Ichimaru, Toru
Sunaga, Fujiko
Mukono, Itsuro
Yamasato, Hiroshi
Shirai, Junsuke
Katayama, Kazuhiko
Mizutani, Tetsuya
Oka, Tomoichiro
Nagai, Makoto
description Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (>6000 nucleotide: nt) and three partial sequences (2429nt, 4364nt, and 4419nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach. Phylogenetic analysis of the complete VP1 amino acid sequence (aa) revealed that 29 porcine SaVs were classified into seven genogroups; GIII (11 strains), GV (1 strain), GVI (3 strains), GVII (6 strains), GVIII (1 strain), GX (3 strains), and GXI (4 strains). This manuscript presents the first nearly complete genome sequences of GX and GXI, and demonstrates novel intergenogroup recombination events. •Nearly complete genome sequences of sapovirus from pigs were identified.•They were classified into seven genogroups.•Nearly complete genome sequences of GX and GXI represent the first report.•Possible intergenogroup recombination event was identified.
doi_str_mv 10.1016/j.meegid.2017.09.013
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source ScienceDirect Journals
subjects Animals
Feces - virology
Genetic Variation
Genogroup
Genome, Viral
High-Throughput Nucleotide Sequencing
Humans
Japan
Pig
Recombination
Recombination, Genetic
Sapovirus
Sapovirus - classification
Sapovirus - genetics
Sapovirus - isolation & purification
Swine
Swine Diseases - epidemiology
Swine Diseases - virology
Viral Proteins - genetics
title Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan
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