Iron deficiency stimulates anthocyanin accumulation in grapevine apical leaves

• Published in: Plant physiology and biochemistry 2017-10, Vol.119, p.286-293
• Main Authors: Caramanico, Leila, Rustioni, Laura, De Lorenzis, Gabriella
• Format: Article
• Language: English
• Subjects: Anthocyanins; Anthocyanins - biosynthesis; Gene expression; Iron - deficiency; Iron - metabolism; Iron chlorosis; Leaf pigmentation; Plant Leaves - metabolism; Vitis - metabolism; Vitis vinifera
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2017.09.004

Iron chlorosis is a diffuse disorder affecting Mediterranean vineyards. Beside the commonly described symptom of chlorophyll decrease, an apex reddening was recently observed. Secondary metabolites, such as anthocyanins, are often synthetized to cope with stresses in plants. The present work aimed to evaluate grapevine responses to iron deficiency, in terms of anthocyanin metabolism (reflectance spectrum, total anthocyanin content, HPLC profile and gene expression) in apical leaves of Cabernet sauvignon and Sangiovese grown in hydroponic conditions. Iron supply interruption produced after one month an increasing of anthocyanin content associated to a more stable profile in both cultivars. In Cabernet sauvignon, the higher red pigment accumulation was associated to a lower intensity of chlorotic symptoms, while in Sangiovese, despite the activation of the metabolism, the lower anthocyanin accumulation was associated to a stronger decrease in chlorophyll concentration. Gene expression data showed a significant increase of anthocyanin biosynthesis. The effects on the expression of structural and transcription factor genes of phenylpropanoid pathway were cultivar dependent. F3H, F3′H, F3′5′H and LDOX genes, in Cabernet sauvignon, and AOMT1 and AOMT genes, in Sangiovese, were positively affected by the treatment in response to iron deficiency. All data support the hypothesis of an anthocyanin biosynthesis stimulation rather than a decreased degradation of them due to iron chlorosis. [Display omitted] •Iron deficiency stimulates anthocyanin metabolism in grapevine apical leaves.•Both the total accumulation and the pigment profiles are affected.•Expression of phenylpropanoid pathway genes is up-regulated under iron deficiency.