Fibrocytes Are Involved in Inflammation as well as Fibrosis in the Pathogenesis of Crohn’s Disease

Background We previously showed that fibrocytes, a hematopoietic stem cell source of fibroblasts/myofibroblasts, infiltrated the colonic mucosa of a murine colitis model. Aim We investigated whether fibrocytes were involved in the pathogenesis of Crohn’s disease. Methods Human surgical intestinal sp...

Full description

Saved in:
Bibliographic Details
Published in:Digestive diseases and sciences 2014-04, Vol.59 (4), p.760-768
Main Authors: Sazuka, Sayuri, Katsuno, Tatsuro, Nakagawa, Tomoo, Saito, Masaya, Saito, Keiko, Maruoka, Daisuke, Matsumura, Tomoaki, Arai, Makoto, Miyauchi, Hideaki, Matsubara, Hisahiro, Yokosuka, Osamu
Format: Article
Language:English
Subjects:
Antibodies
Biochemistry
Cells, Cultured
Colitis
Collagen
Collagen Type I - physiology
Comparative analysis
Crohn Disease - etiology
Crohn Disease - metabolism
Crohn Disease - pathology
Crohn Disease - physiopathology
Fibroblasts - metabolism
Fibroblasts - physiology
Fibrosis
Flow Cytometry
Gastroenterology
Gene Expression
Hematopoietic stem cells
Hepatology
Humans
Immunohistochemistry
Inflammation
Intercellular Adhesion Molecule-1 - metabolism
Intestinal Mucosa - cytology
Intestinal Mucosa - metabolism
Medicine
Medicine & Public Health
Oncology
Original Article
Pneumoviridae
Receptors, CXCR4 - physiology
RNA
Transplant Surgery
Tumor Necrosis Factor-alpha - metabolism
Viral antibodies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background We previously showed that fibrocytes, a hematopoietic stem cell source of fibroblasts/myofibroblasts, infiltrated the colonic mucosa of a murine colitis model. Aim We investigated whether fibrocytes were involved in the pathogenesis of Crohn’s disease. Methods Human surgical intestinal specimens were stained with anti-leukocyte-specific protein 1 and anti-collagen type-I (ColI) antibodies. Circulating fibrocytes in the human peripheral blood were quantified by fluorescence-activated cell sorting with anti-CD45 and anti-ColI antibodies. Cultured human fibrocytes were prepared by culturing peripheral CD14 + monocytes. Results In the specimens of patients with Crohn’s disease, the fibrocyte/total leukocyte percentage was significantly increased in inflammatory lesions (22.2 %, p  
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-013-2813-8