Use of AFLP for the study of eukaryotic pathogens affecting humans

Amplified fragment length polymorphism (AFLP) is a genotyping technique based on PCR amplification of specific restriction fragments from a particular genome. The methodology has been extensively used in plant biology to solve a variety of scientific questions, including taxonomy, molecular epidemio...

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Bibliographic Details
Published in:Infection, genetics and evolution genetics and evolution, 2018-09, Vol.63, p.360-369
Main Authors: Restrepo, Carlos M., Llanes, Alejandro, Lleonart, Ricardo
Format: Article
Language:English
Subjects:
AFLP
Amoebozoa - classification
Amoebozoa - genetics
Amoebozoa - isolation & purification
Amplified Fragment Length Polymorphism Analysis - statistics & numerical data
Animals
Apicomplexa - classification
Apicomplexa - genetics
Apicomplexa - isolation & purification
Eukaryotic pathogens
Fungi
Fungi - classification
Fungi - genetics
Fungi - isolation & purification
Genetics, Population
Genome
Helminths - classification
Helminths - genetics
Helminths - isolation & purification
Humans
Kinetoplastida - classification
Kinetoplastida - genetics
Kinetoplastida - isolation & purification
Molecular Epidemiology
Parasites
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Polymorphisms
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Description
Summary:Amplified fragment length polymorphism (AFLP) is a genotyping technique based on PCR amplification of specific restriction fragments from a particular genome. The methodology has been extensively used in plant biology to solve a variety of scientific questions, including taxonomy, molecular epidemiology, systematics, population genetics, among many others. The AFLP share advantages and disadvantages with other types of molecular markers, being particularly useful in organisms with no previous DNA sequence knowledge. In eukaryotic pathogens, the technique has not been extensively used, although it has the potential to solve many important issues as it allows the simultaneous examination of hundreds or even thousands of polymorphic sites in the genome of the organism. Here we describe the main applications published on the use of AFLP in eukaryotic pathogens, with emphasis in species of the groups fungi, protozoa and helminths, and discuss the role of this methodology in the context of new techniques derived from the advances of the next generation sequencing. •AFLP genotyping technique has not been extensively used on eukaryotic pathogens.•Main published AFLP applications were in organisms of the group Fungi.•In Protozoa, most reports are concerning Trypanosoma and Leishmania species.•AFLP genotyping may complement new markers derived from NGS.
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2017.09.017